70例严重少精症和无精症患者Y染色体微缺失检测  

Detection of Y Chromosome Microdeletions in 70 Infertile Men with Severe Oligozoospermia or Azoospermia

作　　者：王喜良[1] 郝冬梅[1] 侯开波[1] 张宁[1] 闫素文[1] 

机构地区：[1]中国人民解放军第202医院全军计划生育优生优育技术研究所,辽宁沈阳110003

出　　处：《沈阳医学院学报》2010年第3期154-155,共2页Journal of Shenyang Medical College

摘　　要：目的:探讨严重少精症和无精症与Y染色体微缺失的关系。方法:对染色体正常的70例严重少精症和无精症患者运用多重PCR技术检测Y染色体AZF基因家族AZFa、AZFb、AZFc三个区域中6个序列标签位点(sequence taggedsites,STS)微缺失,20例精液正常患者作为对照。结果:39例严重少精症患者中发现5例存在Y染色体微缺失,均为AZFc缺失,缺失率为12.82%(5/39);31例无精症患者中发现6例存在Y染色体微缺失,其中AZFb+AZFc缺失2例,AZFb缺失3例,AZFc缺失1例,缺失率为19.35%(6/31)。20例精液正常患者Y染色体均未发现微缺失。结论:Y染色体微缺失是造成男性精子发生障碍的常见病因之一。Objective：To assess the relationship between severe oligozoospermia or azoospermia and Y chromosomal microdeletions.Methods：Seventy chromosome normal infertile men with severe oligozoospermia or azoopermia were screened by multi-polymerase chain reactions with 6 sequence tagged sites in AZFa、AZFb、AZFc region,and 20 normospermic men as controls.Results：12.82 %（5/39）of microdeletions were detected in 39 severe oligozoospermia,and microdeletion type was AZFc.19.35 %（6/31）of microdeletions were detected in 31 azoospermia,and microdeletion types were AZFb＋AZFc,AZFb and AZFc.Microdeletions were not detected in 20 normospermic men.Conclusion：Y chromosome microdeletion is one of the causes which result in male spermatogenetic obstacle.

关 键 词：严重少精症 无精症 Y染色体微缺失 

分 类 号：R698.2[医药卫生—泌尿科学]