非小细胞肺癌中缺氧诱导因子1α、葡萄糖转运蛋白1和Glypican-3的表达及临床意义研究  

The Expression and Clinical Significance of Hypoxia Induced Factor 1α and Glucose Transporter 1 and Glypican-3 in Non-Small Cell Lung Carcinoma

作　　者：范大平[1] 陈艳昕[2] 张一梅[1] 

机构地区：[1]哈尔滨医科大学附属第一医院呼吸内科,黑龙江省哈尔滨市150001 [2]秦皇岛市第一医院

出　　处：《中国全科医学》2010年第21期2342-2344,2348,共4页Chinese General Practice

基　　金：黑龙江省卫生厅科研课题(2009-097)

摘　　要：目的探讨肺癌组织中缺氧诱导因子-1α(HIF-1α)、葡萄糖转运蛋白1(Glut1)、Glypican-3的表达及其临床意义。方法采用免疫组织化学方法检测52例(男35例,女17例;鳞癌32例,腺癌20例)肺癌组织中HIF-1α、Glut1、Glypican-3的表达。结果 52例肺癌组织中HIF-1α阳性表达36例(69.2%);Glut1阳性表达38例(73.1%);Glypican-3阳性表达7例(13.5%)。肺癌组织Ⅲ～Ⅳ期与Ⅰ～Ⅱ期中高度分化与低分化组比较,HIF-1α、Glut1阳性表达率均明显增加,差异有统计学意义(P<0.05)。肺癌组织有淋巴结转移组与无淋巴结转移组比较,HIF-1α、Glut1阳性表达率均明显增加,差异有统计学意义(P<0.05)。肺癌组织中不同年龄组及不同性别组比较,HIF-1α、Glut1阳性表达差异无统计学意义(P>0.05)。Glypican-3在肺癌组织中与在癌旁组织中阳性表达率差异无统计学意义(P>0.05)。结论肺癌组织中HIF-1α与Glut1蛋白阳性表达,HIF-1α与Glut1阳性表达与肺癌的病理分期、分化程度和淋巴结转移有关,与年龄、性别等无关。Glypican-3在肺癌中表达较低,与癌旁组织中的表达无差异。Objective To explore the expression and clinical significance of hypoxia induced factor 1α（HIF-1α） and glucose transporter 1 （Glut 1） and Glypican-3 in lung carcinoma.Methods The expression of HIF-1αand Glut1 and Glypican-3 were tested by immunohistochemistry in 52 cases of lung cancer specimens （35 males and 17 females;32 cases with squamous carcinoma,20 cases with adenocarcinoma）.Results In 52 cases of lung carcinoma specimens,36 cases showed positive staining of HIF-1α protein （69.2% ）;38 cases showed positive staining of Glut 1 protein （73.1% ）.7 cases showed positive staining of Glypican-3 （13.5% ）.The expression rate of HIF-1a and Glut1 in lung carcinoma tissues of Ⅲ-Ⅳ stage poorly differentiated groups was significantly higher than that inⅠ-Ⅱstage middle-highly differentiated groups （P0.05 ）.The expression of HIF-1a and Glut 1with lymphatic metastasis in lung carcinoma was significantly higher than those without lymphaticmetastasis （P0.05）.The expression rate of HIF-1a and Glut1 in lung carcinoma tissues was not significantly higher in different age groups or different sex groups（P0.05）.The positive expression of Glypican-3 in lung cancer tissues and non-neoplasia lung tissues was low and the difference between the two groups was not statistically singificant（P0.05）.Conclusion High expression of HIF-1αand Glut 1at protein level could be seen in human lung cancer,high expression of HIF-1α was associated with invasion degree and differentiation degree and lymphatic metastasis of lung carcinoma but not with the age or gender of the patients.Low expression of Glypican-3 could be seen in human lung cancer,the difference of expression in lung cancer and in non-neoclassic lung tissues was not significant.

关 键 词：肺肿瘤 单糖转运蛋白质类 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]