利用响应曲面法优化γ-亚麻酸-β-环糊精包合物制备工艺的研究  

Optimization of Preparation of the γ-Linolenic Acid-β-Cyclodetrin Inclusion Complex by Response Surface Methodology

作　　者：孔秀芹[1] 候丽娟[2] 葛海涛[2] 刘志礼[2] 殷志敏[1] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210046 [2]南京大学生命科学学院,江苏南京210093

出　　处：《安徽农业科学》2010年第20期10903-10906,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]优选β-环糊精包合γ-亚麻酸的最佳工艺。[方法]采用BOX-Behnken设计和响应曲面分析优选包合的最佳工艺,并采用差示扫描量热分析及X-射线衍射对包合物进行定性分析。[结果]β-环糊精包合γ-亚麻酸的最佳工艺条件为:β-环糊精与γ-亚麻酸的物质的量比6.47∶1、温度51.15℃、包合时间2.03h,在该条件下包合率达91.51%。定性分析表明γ-亚麻酸与β-环糊精确实形成了包合物,其包合物的结构与简单混合物的结构有明显不同,已构成新的固体相。[结论]该研究可有效地减少工艺操作的盲目性,为进一步的放大生产提供依据。[Objective]To optimize the conditions for the preparation of the γ-linolenic acid-β-cyclodetrin inclusion complex.[Method]Response surface methodology based on a three-factor Box-Behnken design of experiments was used to investigate the optimal conditions.The characterization of the inclusion complex was confirmed by differential scanning calorimetry and X-ray diffraction techniques.[Results]The optimal conditions of encapsulation were as follows：the ratio of β-CD to γ-linolenic acid 6.47∶1,inclusion temperature 51.15 ℃,inclusion time 2.03 h.Under these conditions,the inclusion rate of γ-linolenic acid was up to 91.51%.The study of the characterization of the inclusion complex showed that there was the evident difference between the structure of the inclusion complex and the mixture of the two materials.A new solid matter was constituted after inclusion.[Conclusion]This study could reduce the blindness in operation and provide basis for the scale-up production of γ-linolenic acid-β-cyclodetrin inclusion complex.

关 键 词：Γ-亚麻酸 Β-环糊精 包合物 响应曲面法 

分 类 号：R283.2[医药卫生—中药学]