阿魏酸与DNA相互作用的光谱电化学研究  

Electrochemical and Spectral Study on the Interaction between Ferulic Acid and DNA

作　　者：项朋志[1] 刘丽梅[1] 郭英[2,3] 高云涛[2] 王辰[2] 

机构地区：[1]云南国防工业职业技术学院,云南昆明650222 [2]云南民族大学化学与生物技术学院,云南昆明650031 [3]郧阳师范高等专科学校化学系,湖北丹江口442700

出　　处：《化学与生物工程》2010年第9期53-56,共4页Chemistry & Bioengineering

基　　金：云南省教育厅科学研究基金资助项目(08C0283)

摘　　要：采用电化学分析法研究了阿魏酸与DNA间的相互作用。电化学实验结果表明,DNA的存在使阿魏酸的氧化峰电流减小,且峰电位正移。紫外吸收光谱分析表明,DNA的存在使阿魏酸在310 nm处的吸收峰强度降低,呈减色效应,且出现两个等电吸收点,说明阿魏酸与DNA的相互作用以嵌插作用为主。通过计算,双链DNA(dsDNA)与阿魏酸的结合数约为1,结合常数为1.01×104。The interaction between ferulic acid and DNA was studied by electrochemical method and UV absorption spectrometry.The results indicated that the oxidation peak currents of ferulic acid decreased and the peak potentials shifted positively with adding DNA.UV Absorption spectrum analysis showed that with the addition of DNA,the absorption peak of ferulic acid at 310 nm decreased presenting hypochromic effect,and two isoelectric absorption points appeared.The results indicated that ferulic acid reacted with DNA mainly in a mode of intercalation.By calculation,it was found that the combining number of ferulic acid to dsDNA was 1 and the combining constant was 1.01×104.

关 键 词：阿魏酸 DNA 循环伏安法 紫外吸收光谱 相互作用 

分 类 号：O657.3[理学—分析化学]