昆明产银杏叶总黄酮含量测定及抗氧化活性研究  

Studies on Antioxidation and Determination of Total Flavonoids in Ginkgo Biloba Leaves from Kunming

作　　者：黄相中[1] 尹燕 杨晴来[1] 刘晓芳[1] 梁辉[1] 

机构地区：[1]云南民族大学化学与生物技术学院云南民族药资源化学国家民委-教育部重点实验室,云南昆明650031 [2]云南瑞宝生物科技有限公司,云南昆明650217

出　　处：《云南民族大学学报（自然科学版）》2010年第5期369-371,共3页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：教育部科技研究重点项目(209116)

摘　　要：目的:测定昆明产银杏叶提取物中总黄酮的含量和研究其抗氧化活性.方法:采用AlCl3显色分光光法测定银杏叶提取物中总黄酮的含量,并利用DPPH自由基(DPPH.)捕获分光光度法和连苯三酚红褪色光度法对银杏叶提取物进行抗氧化活性测定.结果:银杏叶提取物中总黄酮含量为5.85%;提取物对DPPH.的捕获能力达59.11%,IC50=114.6μg/mL,对.OH的最大清除率为39.16%.结论:昆明产银杏叶提取物具有良好的抗氧化能力.This research aimed to determine the total flavonoids content in the extract of Ginkgo biloba leaves from Kunming and studied its antioxidant activities. AlCl3 spectrophotometric method was used to determine the content of the total flavone in this extract. Two photometric methods were used to determine its antioxidant activities. The results have shown that the content of the total flavonids in G. biloba leaf extract is 5.85% , its DDPH free radical scavenging activity is 59. 11%, its IC50 is 114.6 μg/mL, its hydrogen free radical scavenging activity is 39. 16%. Thus, Ginkgo biloba leaf extract has good antioxidant activities.

关 键 词：银杏叶 黄酮 抗氧化 

分 类 号：K282.71[历史地理—历史学]