口腔螺旋体的胶原结合蛋白  

Collagen binding proteins of human oral spirochetes

作　　者：李鸣宇[1,2] 刘正梅 本利彦 

机构地区：[1]上海第二医科大学口腔医学研究所 [2]日本朝日大学口腔细菌学教研室

出　　处：《中华口腔医学杂志》1999年第3期165-167,共3页Chinese Journal of Stomatology

摘　　要：目的研究口腔螺旋体胶原结合蛋白的致病作用。方法用免疫印迹、免疫金标电镜和体外粘附实验等方法分析了口腔螺旋体胶原结合蛋白的性质及其在菌细胞表面的定位。结果Ｔｒｅｐｏｎｅｍａｄｅｎｔｉｃｏｌａ菌（简称Ｔｄ）细胞外膜上有２７０００Ⅴ型，Ｔｒｅｐｏｎｅｍａｓｏｃｒａｎｓｋｉ菌（简称Ｔｓ）有９５０００Ⅳ型和１１００００Ⅰ型，Ｔｒｅｐｏｎｅｍａｍｅｄｉｕｍ（简称Ｔｍ）有９５０００Ⅳ型胶原结合蛋白；Ｔｄ和Ｔｓ可粘附于Ⅳ型胶原表面，而Ｔｍ很少粘附；Ｔｄ和Ｔｍ可粘附于无胶原蛋白涂布的薄片表面。结论胶原结合蛋白可介导螺旋体对胶原较丰富的靶器官的粘附。Objective To evaluate pathogenic effect of the collagenbinding proteins(CBP) of human oral spirochetes. Methods Immunoblot analysis, immunoelectron microscopy and other experiments had been used. ResultsT5BZIt was showed that: type , and CBP were detected in heated and unheated preparations from Treponema denticola(Td) ATCC 33520 and Treponema socranskii (Ts) ATCC 35534. Few CBP, however, were detected in heated and unheated preparations from a recently characterized isloate, Treponema medium(Tm) strain G7201. 27 000 type CBP of Td, 110 000 typeof Ts and 95 000 type of Tm were located on the envelopes of the individual cells. The adherence of Td to the collagencoated surface was significantly greater than that of Tm. HZConclusion The CBP on the oral spirochetal cells play an important role in their adherence to collagenrich connective tissues of the host.

关 键 词：螺旋体属 细菌粘附 胶原 口腔螺旋体 

分 类 号：R780.2[医药卫生—口腔医学]