聚乳酸和骨生长因子复合植入块修复骨缺损的实验研究  

Polylactic acid combined with recombinant human bone morphogenetic protein and basic fibroblast growth factor to repair the mandibular defects in rabbits

作　　者：许建辉[1,2] 孟昭业[1,2] 杨震[1,2] 张森林[1,2] 梁河清[1,2] 

机构地区：[1]南京军区南京总医院口腔科 [2]第一军医大学珠江医院口腔科

出　　处：《中华口腔医学杂志》1999年第3期168-171,共4页Chinese Journal of Stomatology

摘　　要：目的探讨碱性成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ）对重组人骨形成蛋白２（ｒｅｃｏｍｂｉｎａｎｔｈｕｍａｎｂｏｎｅｍｏｒｐｈｏｇｅｎｅｔｉｃｐｒｏｔｅｉｎ２，ｒｈＢＭＰ２）诱导骨形成的影响，评价多孔聚乳酸（ｐｏｌｙｌａｃｔｉｃａｃｉｄ，ＰＬＡ）复合人工骨修复骨缺损的作用。方法在５６例下颌骨缺损的兔模型中分别植入ＰＬＡ／ｒｈＢＭＰ２／ｂＦＧＦ、ＰＬＡ／ｒｈＢＭＰ２、ＰＬＡ／ｂＦＧＦ、ＰＬＡ，并设空白对照。植入后２、４、８周行Ｘ线摄片和组织学观察骨缺损区骨形成的情况。结果３种材料复合物较两种材料复合物成骨更好，而后者又较单一的ＰＬＡ成骨好，所有含骨生长因子的植入组成骨量与对照组比较差异均有显著性（Ｐ＜０．０５）。结论ＰＬＡ具有良好的生物相容性和可吸收性，有骨传导性能；ｂＦＧＦ可促进ｒｈＢＭＰ２在骨缺损愈合过程中的骨诱导作用；ＰＬＡ／ｒｈＢＭＰ２／ｂＦＧＦ复合材料是一种有临床应用前景的骨移植材料。Objective To study the effect of basic fibroblast growth factor(bFGF) on bone formation induced by recombinant human bone morphogenetic protein2(rhBMP2) in repair of mandibular defects in rabbits. Methods 56 adult domestic rabbits were classified into five groups. The mandiblar threewall defects(1.5 cm0.5 cm ,full thick) were created in right sides. The defects were implanted with various grafts: PLA/rhBMP2/bFGF, PLA/rhBMP2, PLA/bFGF, PLA and no implant as control group. At 2, 4 and 8 weeks after operation, the effectivenesses of defect repaire were observed by histological and roentgenographic analysis. Results The composite consisting of three materials stimulated more bone formation than of two materials, and the latter was more than single PLA(at 4 weeks postoperation, P<0.05). All experimental groups,which had one or two growth factors, were significantly higher compared with control group in every stage of postimplantation. Conclusion PLA has good biocompatibility, absorbability and osteoconductibility, rhBMP2 and bFGF are cooperated in bone healing process, and PLA/rhBMP2/bFGF is a promising bone substitute in clinical uses.

关 键 词：重组蛋白质类 骨基质 BFGF 骨缺损 聚乳酸 

分 类 号：R782.4[医药卫生—口腔医学] R783.1[医药卫生—临床医学]