影响血链球菌粘附因素的研究  

Regulation of adherence to serumcoated hydroxyapatite by Streptococcus sanguis

作　　者：宋晓波[1,2,3] 潘亚萍[1,2,3] 孔庆华 康白[1,2,3] 

机构地区：[1]大连医科大学微生态研究所 [2]中国医科大学附属口腔医院 [3]大连市口腔医院

出　　处：《中华口腔医学杂志》1999年第3期172-174,共3页Chinese Journal of Stomatology

摘　　要：目的探讨ｐＨ值、钙离子对血链球菌粘附至血清，包被羟磷灰石的影响。方法采用Ｃｌａｒｋ创立的体外粘附实验模型，以［３Ｈ］胸腺嘧啶核苷为标记对血链球菌的粘附进行定量观察。结果不同ｐＨ值组测得的放射性测量计数率（ｃｏｕｎｔｓｐｅｒｍｉｎｕｔｅ，ｃｐｍ）差异有显著性；其中ｐＨ值为６．０～７．５时ｃｐｍ值最高，ｐＨ值小于５．５时ｃｐｍ值则明显降低。不同钙离子浓度的实验组ｃｐｍ值均显著高于对照组，当钙离子浓度为１．２５～３．７５ｍｍｏｌ／Ｌ时ｃｐｍ值最高。结论ｐＨ值、钙离子对血链球菌的粘附作用有显著影响，调节牙周局部ｐＨ值及钙离子浓度可影响血链球菌在牙周环境的定植。Objective To investigate the effects of pH and calcium iron on adhesion of Streptococcus sanguis to serumcoated hydroxyapatite. HZMethods The adhesion model in vitro established by Clark W.B. in 1977 was used to quantify adsorptive cells through thymidine labelling. Results5BZThe cpm values between different pH groups showed significant differences. Also, there were significant differences of cpm values between calcium groups and control group. HZConclusion Either pH or calcium iron has obvious effect on adherence of Stroptococcus sanguis to serumcoated hydroxyapatite. The findings suggest that regulating pH and concentration of calcium iron can help to change colonization on teeth surfaces by Streptococcus sanguis in periodontal circumstance.

关 键 词：链球菌 血 细菌粘附 氢离子浓度 钙 牙周病 

分 类 号：R781.402[医药卫生—口腔医学]