药对白术-茅苍术及其单味药中挥发油成分的比较分析  

Comparative Analysis of Volatile Constituents in Herbal Pair Atractylodes Macrocephala-Atractylodes Lancea and Its Single Herb

作　　者：佘金明[1] 梁逸曾[1] 彭友林[2] 华美铃[1] 谭斌斌[1] 李洪东[1] 

机构地区：[1]中南大学化学化工学院,中药现代化研究中心,长沙410083 [2]湖南文理学院生命科学系,常德415000

出　　处：《分析化学》2010年第9期1282-1286,共5页Chinese Journal of Analytical Chemistry

基　　金：科技部国际合作项目(No2007DFA40680)资助

摘　　要：采用水蒸气蒸馏法提取单味药白术、茅苍术及其药对中挥发油成分,通过气-质联用技术(GC-MS)对其分离检测,利用直观推导式演进特征投影法(HELP)分辨解析重叠色谱峰,并结合程序升温保留指数辅助定性。从白术、茅苍术及其药对中依次鉴定出29,50,62个组分,分别占各自挥发油总量的95.93%,97.44%,97.47%;其中20种挥发油成分共存于单味药与药对中,主要是2-(2-甲氧基)苯甲氧基苯酚、γ-芹子烯、雅槛蓝树油烯、大根香叶烯B等。组成药对后,白术减少8种组分,茅苍术减少12种组分,而药对新增22种组分,主要是单萜烯类的低沸点化合物,新增组分在药对中的作用不容忽视。The volatile constituents in the single herb Atractylodes macrocephala（AM）,Atractylodes lancea（AL） and their herbal pair（HP） were extracted by steam distillation.With temperature-programmed retention index for assistant identification,gas chromatography-mass spectrometry（GC-MS） combined with heuristic evolving latent projections（HELP）,was used to identify and analyze the volatile constituents comparatively.29,50 and 62 kinds of constituents of the essential oils were identified from AM,AL and its HP respectively,and the relative amount accounted for 95.93%,97.44% and 97.47% of the total relative contents in order.There were 20 volatile constituents in common in single herb and HP,mainly including 2-（2-methoxyphe-noxy） phenol,γ-selinene,eremophilene,germacrene B,3,7（11）-selinadiene,elixene,and so on.After the formation of the HP,8 kinds of constituents in the single herb AM and 12 components in the AL were reduced,while 22 additional components increased in the HP.The new additional components mainly belonged to monoterpene with low boiling point,and they played an important role in the HP.

关 键 词：药对白术-茅苍术 挥发油 气相色谱-质谱 直观推导式演进特征投影法 

分 类 号：R284[医药卫生—中药学]