牦牛和黄牛三个微卫星基因座种间特异性等位基因分析  

Species specific alleles at three microsatellite loci in yak and cattle

作　　者：冯冬梅[1,2] 赵会静[2,3] 罗玉柱[1] 韩建林[2,3] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]中国农业科学院北京畜牧兽医研究所,农业部畜禽遗传资源与利用重点开放实验室,北京100193 [3]中国农业科学院北京畜牧兽医研究所,国际家畜研究所,畜禽牧草遗传资源联合实验室,北京100193

出　　处：《甘肃农业大学学报》2010年第4期22-27,共6页Journal of Gansu Agricultural University

基　　金：“十一五”国家科技支撑计划项目(2007BAD52B05);CAAS-ILRI联合研究项目;中国农业科学院一级岗位杰出人才基金

摘　　要：采用PCR产物直接测序和克隆测序相结合的方法分析了牦牛、普通牛和瘤牛在ILSTS013、ILSTS050和SPS115 3个微卫星基因座上存在的特异性等位基因座位的特异性.结果表明:牦牛、普通牛和瘤牛在这3个基因座上等位基因的DNA序列存在种间特异性差异,依据这些特点可以将牦牛与普通牛和瘤牛在基因型或DNA序列水平上进行区分,这将成为评价牛种间的基因交流水平和建立牛肉及其产品分子追溯系统的可靠分子标记.The species SPS115 were verified using sp di ecific alleles at three microsatellite DNA loci of ILSTS013, ILSTS050 and rect PCR product sequencing and/or cloning sequencing methods. The results confirmed that yak, taurine and indicine cattle had different sequence characteristics to each other in the al leles at these three loci,leading to species specific allele sizes in genotyping exercise. Therefore these three loci can be used for discriminating alleles originated from yak, taurine and indicine cattle in assessment of the level of genetic admixture between bovine species and in establishment of molecular traceability systems for beef and its products from different bovine species.

关 键 词：牦牛 黄牛 微卫星DNA标记 物种特异性等位基因 

分 类 号：S823[农业科学—畜牧学]