诱导体外扩增的小鼠骨髓CD34^+造血细胞分化为树突状细胞  

INDUCTION OF DENDRITIC CELL DIFFERENTIATION FROM MURINE BONE MARROW CD34 + HEMATOPOIETIC CELLS EXPANDED EX VIVO

作　　者：董强刚[1,2] 叶闻斐 王亦宾 江晓丰[1,2] 冯久贤 

机构地区：[1]上海市胸科医院 [2]上海市胸部肿瘤研究所

出　　处：《肿瘤》1999年第3期132-136,共5页Tumor

基　　金：国家自然科学基金;上海市卫生系统"百人计划"资助

摘　　要：诱导体外培养扩增后的小鼠骨髓ＣＤ３４＋造血细胞分化为树突状细胞（ＤＣ）。方法Ｃ５７ＢＬ／６ｊ小鼠骨髓ＣＤ３４＋细胞采用血管内皮细胞共培养方法进行体外扩增，扩增后的ＣＤ３４＋细胞经磁性细胞分选技术纯化，并用ＧＭ┐ＣＳＦ等细胞因子诱导其向ＤＣ分化。结果小鼠骨髓ＣＤ３４＋造血细胞不论是否经过体外培养均可被诱导分化为ＤＣ，后者具有典型的树突状形态特征，表达高水平的ＭＨＣⅠ、Ⅱ类抗原和ＣＤ８６共刺激分子，在混合白细胞反应（ＭＬＲ）中能有效地刺激静息期Ｔ淋巴细胞增殖。结论小鼠骨髓ＣＤ３４＋细胞经体外培养扩增后仍然具有向ＤＣ分化的能力，通过骨髓细胞体外培养扩增可以显著提高ＤＣ产量。Objective:To induce the dendritic cell (DC) differentiation from murine bone marrow CD34 + hematopoietic cells expanded ex vivo.Methods:The marrow CD34 + cell of C37BL/6j mice were expanded ex vivo by using a bone marrow microvessel endothelial cell co culture method.The expanded CD34 + cells were purified by MACS and then induced by cytokines including GM CSF to differentiate into DC.Results:The murine bone marrow CD34 + cells can be induced in vitro for DC differentiation,regardless of expanded ex vivo or not.The obtained cells had a typical morphology constant to DC,expressed a high level of MHC class Ⅰ and Ⅱ antigens as well as CD86 co stimulator molecule,and were potent stimulators for quiescent T lymphocytes in the mixed leukocyte reaction.Furthermore,the expanded marrow cells can be induced directly into DC without the need of purification procedure,and thereby the yield of DC can be further increased.Conclusion:Murine marrow CD34 + cells after expanded ex vivo remained the capacity of differentiating into DC.The yield of DC can be, therefore, increased significantly by ex vivo expansion of the marrow hematopoietic cells.

关 键 词：树突细胞 CD34 造血干细胞 体外扩增 生物治疗 

分 类 号：R730.51[医药卫生—肿瘤] R730.21[医药卫生—临床医学]