原发性皮肤T细胞淋巴瘤外周血克隆性T细胞的检测与研究  

Detection fo clonal T cell in peripheral blood and its correlation with therapeutic effect in primary cutaneous T cell lymphoma

作　　者：王平[1,2,3] 邱丙森[1,2,3] 陈明华 高红阳[1,2,3] 单易非 

机构地区：[1]南京铁道医学院第一附属医院皮肤科 [2]上海医科大学皮肤病学研究所病理研究室 [3]上海医科大学分子生物物理教研室

出　　处：《临床皮肤科杂志》1999年第3期156-158,共3页Journal of Clinical Dermatology

摘　　要：为了解原发性皮肤Ｔ细胞淋巴瘤（ＰＣＴＣＬ）外周血中克隆性Ｔ细胞的存在及其与临床疗效的相关性，分别对２５例ＰＣＴＣＬ患者经重组α干扰素治疗前后的外周血标本，应用聚合酶链反应（ＰＣＲ）方法，以通用引物扩增Ｔ细胞受体（ＴＣＲ）γ链编码基因中Ｖ（可变区）Ｊ（连接区）的结合序列，检测代表克隆性Ｔ细胞分子标志的ＴＣＲ基因重排（ＧＲ）。结果发现，２５例ＰＣＴＣＬ外周血样品中，１７例见克隆性Ｔ细胞（示ＴＣＲＧＲ），经有效治疗后减至３例，治疗前后克隆性Ｔ细胞的检出率具显著性差异。说明克隆性Ｔ细胞在ＰＣＴＣＬ外周血中常见，外周血中克隆性Ｔ细胞的动态监测对ＰＣＴＣＬ临床疗效的判定有辅助作用。In order to investigate the presence of clonal T cell in peripheral blood of primary cutaneous T cell lymphoma(PCTCL) and its correlation with therapeutic effect. We analysed peripheral blood samples from a total of 25 patients with PCTCL before and after treatment with recombinant interferon alfa. Universal primers were used to amplify the junctional Ⅴ(variable) J(joining) sequences of T cell receptor γ(TCRγ) genes to detect clonal gene rearrangement (GR) by polymerase chain reaction (PCR). The results showed that TCRGR detection before amd after treatment presented statistically significant difference. This suggests that clonal T cell in peripheral blood with PCTCL is common. Dynamic monitoring of clonal T cell in peripheral blood is helpful to assess the therapeutic effect in PCTCL.

关 键 词：T细胞淋巴瘤 克隆性T细胞 皮肤肿瘤 PCR 

分 类 号：R739.5[医药卫生—肿瘤]