TNF-α在大鼠暂时性局灶性脑缺血损伤中的变化  

Changes of turnor necrosis factor-α in the rat brain following transient focal cerebral ischemia

作　　者：叶建锋[1] 李涛平[2] 舒斯云[3] 高宁 

机构地区：[1]广州第一军医大学基础部,510515 [2]第一军医大学南方医院 [3]第一军医大学珠江医院

出　　处：《中国急救医学》1999年第6期329-330,共2页Chinese Journal of Critical Care Medicine

摘　　要：目的用动脉内置线阻断法建立大鼠大脑中动脉（MCA）局灶性脑缺血模型，观察缺血脑区中TNF－α的变化。方法改良MCA缺血模型，设30min缺血后不同时点（6h，12h，24h，48h）再灌注组，采用TNF－α酶联免疫吸附法检测各缺血脑区的TNF水平。结果缺血30min后各时相点中，6h再灌流组缺血侧纹状体和皮层TNF－α水平即明显增加，后略下降，至再灌流48h后出现第2个高峰。结论30min缺血后各时相点的TNF－α出现观相性增高现象。Objective to examine the relase of TNF - a in ischemic cerebrum using reversible focal middle cerebral artery (MCA)ischemia in rat induced by intraluminal vascular occlusion method .Methods 6, 12,24,48 hours reperfusion following 30 -min occlusion using improved focal cerebral ischemia model ,the levels of TNF - a in ischemic cerebrum were detected with Tumor Necrosis Factor-a ELISA Kit.Results The levels of TNF - a in ischemic striatum and cortex obvious increased at 6 hours after 30 - min ischemia and then and then declined . However,it had a second peak following 48 hours reperfusion .Conchusion Increase of the TNF - a displayed biphasic changes in different reperfusion time after 30 - min ischemia .

关 键 词：脑缺血 肿瘤坏死因子 大鼠 MCA 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学]