增生性玻璃体视网膜病变玻璃体切割物的细胞凋亡观察  

The observation on apoptosis from vitrectomy specimens of proliferative vitreoretinopathy

作　　者：杨桦[1] 管红兵[1] 张皙[1] 陈伟[1] 吴乃川[1] 顾青[1] 

机构地区：[1]上海市第一人民医院眼科

出　　处：《中华眼底病杂志》1999年第2期81-83,共3页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的观察增生性玻璃体视网膜病变玻璃体切割物的细胞凋亡情况。方法收集６０例不同分级的增生性玻璃体视网膜病变患者的玻璃体切割物，核苷酸末端转移酶介导ｄＵＴＰ缺口翻译法（ＴｄＴ－ｍｅｄｉａｔｅｄｄＵＴＰｎｉｃｋｅｎｄｌａｂｅｌｉｎｇｍｅｔｈｏｄ，ＴＵＮＥＬ法）标记凋亡细胞，光镜观察。结果６０例患者的玻璃体切割物均有细胞凋亡的特征性改变，随着病变程度的加重，非色素细胞凋亡总数逐渐减少，并出现色素细胞凋亡。结论增生性玻璃体视网膜病变存在不同类型细胞的凋亡，细胞凋亡是调控其病变程度的重要机制之一。urpose To investigate apoptosis in vitrectomy specimens of proliferative vitreoretinopathy. Methods Vitrectomy specimens from 60 cases of different classes of proliferative vitreoretinopathy were studied by TdTmediated dUTP nick end labelling(TUNEL)method. Results The characteristic change of apoptosis was observed in all vitrectomy specimens.The amount of apoptotic nonpigmentary cell is gradually decreasing along with the development of proliferative vitreoretinopathy,and apoptotic pigmentary cells are observed. Conclusion There are different kinds of apoptosis cell in vitrectomy specimens of proliferative vitreoretinopathy.It is suggested that apoptosis might be one of the important mechanisms of regulating the degree in proliferative vitreoretinopathy.

关 键 词：玻璃体 视网膜病变 增生性 细胞凋亡 

分 类 号：R774.120.2[医药卫生—眼科]