宫颈癌组织中p-ERK和TGF—β1蛋白的表达及其相关性  

Expression of external-signal regulated kinase and TGF-β1 protein in human cervical carcinoma and their relationship

作　　者：陈文殊[1] 吕杰强[2] 徐峰[1] 薛海燕[1] 沈旭娜 冯泽蛟[1] 

机构地区：[1]温州医学院定理临床学院妇产科,325000 [2]温州医学院附属第二医院妇产科

出　　处：《浙江医学》2010年第8期1177-1179,1283,共4页Zhejiang Medical Journal

基　　金：温州市科技计划项目（Y20090303）

摘　　要：目的探讨磷酸化的细胞外信号调节激酶（P—ERK）和转化生长因子-β1（TGF-β1）蛋白在宫颈癌组织中的表达及其相关性。方法采用免疫组织化学SP法检测P～ERK和TGF－β1蛋白在宫颈癌（宫颈癌组，n=50）、宫颈上皮内瘤变（CIN组，n=25）和正常宫颈组织（正常宫颈组，n=25）中的表达情况，并分析P—ERK和TGF－β1的相关性。结果P—ERK蛋白在宫颈癌组、CIN组和正常宫颈组中的阳性表达率分别为84％、48％、20％，宫颈癌组与CIN组、正常宫颈组比较差异有显著统计学意义（均P〈O．01），后两组比较差异有统计学意义（P〈O05）。TGF－β1蛋白在宫颈癌组、CIN组和正常宫颈组中的阳性表达率分别为86％、44％、12％，宫颈癌组与CIN组、正常宫颈组比较差异均有显著统计学意义（P〈0．01），后两组比较差异有统计学意义（P〈O．05）。P—ERK和TGF－β1蛋白在宫颈癌组织中的表达呈显著正相关（r=0．457，P〈0.01）。结论P—ERK、TGF－β1与宫颈癌发生有关，两者在宫颈癌组织中的表达呈明显正相关。Objective To investigate the expression of external-signal regulated kinase （ERK）and transfer growth factor-β1 （TGF-β1） in human cervical carcinoma and their relationship. Methods The expressions of ERK and TGF-β1 proteins were detected by immunohistochemical technique in 50 cases of cervical carcinoma, 25 cases of cervical intraepithelial neoplasia （CIN） and 25 samples of normal cervical tissue; and their clinical significance was analyzed. Results The positive rates of ERK and TGF-β1 in cervical carcinoma, CIN and normal cervical tissue were 84% and 86%; 48% and 44%; 20% and 12% respectively. There were significant differences in ERK and TGF-β1 expressions between cervical carcinoma and CIN or normal cervical epithelium （P〈0.01）. There were also significant differences in ERK and TGF-β1 expressions between CIN and normal cervical epithelium（P〈0.05 ）, The expression of ERK was positively correlated with TGF-β1 in human cervical carcinoma （r=0.457, P〈0.01 ）. Conclusion ERK and TGF- β1 are highly expressed in cervical cancer and correlated with each other; both proteins may be involved in the pathogenesis of cervical carcinoma.

关 键 词：宫颈癌 细胞外信号调节激酶 转化生长因子-Β1 免疫组织化学 

分 类 号：R737.14[医药卫生—肿瘤]