原发性肺癌线粒体DNA控制区微卫星不稳定性的研究  

Mitochondrial Microsatellite Instability of Mitochondrial DNA Control Region in Lung Cancer

作　　者：戴纪刚[1] 张在永[1] 肖颖彬[1] 闵家新[1] 张国强[1] 吴秋平[1] 余祖斌[1] 

机构地区：[1]第三军医大学新桥医院,重庆400037

出　　处：《中国肿瘤》2010年第9期604-607,共4页China Cancer

基　　金：国家自然科学基金(39900173);重庆市自然科学基金资助(2009C195)

摘　　要：[目的]探讨线粒体DNA控制区微卫星不稳定性(mitochondrial microsatellite instability,mtMSI)与肺癌的相关性。[方法]采用PCR-SSCP(PCR single-stranded conformation polymorphism,PCR-SSCP)和序列测定技术对37例肺癌组织及癌旁组织线粒体DNA控制区的3个微卫星位点进行了分析。[结果]37例肺癌样本中共检出mtMSI12例(32.4%),肺癌mtMSI发生率与患者性别、年龄及肿瘤的病理类型无相关性(P>0.05),吸烟组(包括曾经吸烟者)与非吸烟组mtMSI发生率两者有显著差异(P<0.05)。[结论]线粒体DNA控制区的遗传不稳定性在一些肺癌的发生中可能起一定作用。[Purpose]To investigate the mitochondrial microsatellite instability（mtMSI）of mitochondiral DNA control region（D-loop）in lung cancer and its relation with lung cancer.[Methods]Mitochondrial DNA D-loop of 37 matched lung cancer and adjacent normal lung tissue samples were analyzed by PCR-SSCP and sequencing technique.[Results]Mitochondrial DNA mtMSI occurred in 32.4%（12/37）of Lung cancer samples.Incidence of mtMSI in lung cancer did not correlate to agender,age and pathological type of tumor（P0.05）.Incidence of mtMSI was significantly different between smoking group（including smoked people）and no-smoking group（P0.05）.[Conclusion]mtMSI might play an important role in carcinogenesis in some lung cancers.

关 键 词：线粒体DNA 肺癌 微卫星不稳定性 

分 类 号：R734.2[医药卫生—肿瘤]