热休克大鼠小脑中小脑肽1和孤独谷氨酸受体2表达变化及其与血管活性物质相关性研究  

Expression of Cerebellin-1 and Orphan Glntamate Receptor-2 in Heat Shock Rat Cerebellum and Their Correlation with Vasoactive Substances

作　　者：彭森[1,2] 唐忠志[2] 

机构地区：[1]武汉大学医学院病理生理学教研室,湖北武汉430071 [2]中国人民解放军广州军区武汉总医院急诊科,湖北武汉430070

出　　处：《武汉大学学报（医学版）》2010年第5期613-615,618,共4页Medical Journal of Wuhan University

摘　　要：目的:探讨热休克大鼠小脑小脑肽1(Cbln1)和孤独谷氨酸受体-2(Glud2)表达的变化及意义。方法:60只SD大鼠随机分为3组:空白组,休克早期组和休克晚期组。大鼠置于恒温恒湿箱模拟湿热天气。生理记录仪检测大鼠生命体征。ELISA检测小脑组织中Cbln1和血清中降钙素基因相关肽(CGRP)含量,流式细胞仪检测Glud2表达,放射免疫分析法检测血清中肾上腺素(E)、去甲肾上腺素(NE)、血管紧张素-Ⅱ(AngⅡ)、肾素(RA)浓度。结果:休克早期组和休克晚期组Cbln1和Glud2含量显著高于空白组(P<0.05);休克晚期组Cbln1和Glud2含量显著高于休克早期组(P<0.05);相关性分析显示Cbln1、Glud2分别与E、NE、AngⅡ、RA呈正相关(P<0.05);Cbln1和Glud2与CGRP无明显相关性(P>0.05)。结论:热休克大鼠Cbln1和Glud2表达增加,它们可能是自身抗休克机制之一。Objective：To investigate the expressing of Cerebellin-1（Cbln1）and orphan glutamate receptor-2（Glud 2） in the heat shock rat cerebellum.Methods：Sixty SD rats were randomly divided into control group,early shock group and late shock group.Rats were placed in constant temperature and humidity boxes to simulate the weather of hot and humid.Electrophysiolograph detect the vital sign of the rats.Radioimmunoassay was used to detect the serum level of adrenaline （E）,norepinephrine（NE）,angiotensin-Ⅱ （AngⅡ）,and renin（RA）,ELISA was used to determinate the level of Cbln1in the cerebellum and the serum level of calcitonin gene-related peptide （CGRP）,Glud2 expression was detected by flow cytometry.Results：Expression of Cbln1and Glud2 in the early shock group and late shock group were significantly higher than that in the control group respectively（all P0.05）.Expression of Cbln1 and Glud2 in the late shock group were significantly higher than that in the early shock group（both P0.05）.Correlation analysis in Cbln1and Glud2were positively correlated with E,NE,AngⅡ,and RA （P0.05）,but no correlation with CGRP（P0.05）.Conclusion：The over expression of Cbln1and Glud2in heat shock rats may be one of its mechanism of anti-shock.

关 键 词：小脑肽1 孤独谷氨酸受体-2 热休克 

分 类 号：R363.1[医药卫生—病理学]