木耳硒多糖的分离纯化及对腹水型S_(180)细胞膜流动性的影响  

Selenium polysaccharides from Auricularia auricula-judae and their effect on membrane mobility of ascite S_(180) tumor cell

作　　者：宋广磊 杜琪珍 

机构地区：[1]浙江工商大学食品与生物工程研究所,杭州310035

出　　处：《菌物学报》2010年第5期713-718,共6页Mycosystema

基　　金：国家"863计划"专题(No2007AA10Z342)

摘　　要：应用DEAE52-cellulose、SephacrylS-500HR柱层析从自培养的富硒木耳中分离纯化得到4种木耳硒多糖,SeAPⅠ-1、SeAPⅠ-2、SeAPⅡ和SeAPⅢ,紫外扫描显示在280nm和260nm均无吸收,表明不含蛋白质和氨基酸;高效凝胶渗透色谱为单一对称峰,表明4种硒多糖均为均一多糖。对S180细胞膜的游离脂肪酸和膜脂流动性的研究显示,4种硒多糖具有较好的活性,且SeAPⅠ-2、SeAPⅡ的活性显著强于SeAPⅠ-1、SeAPⅢ(p<0.05)。Four selenium polysaccharides, SeAPⅠ-1, SeAPⅠ-2, SeAPⅡ a nd SeAPⅢ were isolated from Auricularia auricula-judae grown in selenium medium by DEAE52-cellulose and Sephacryl S-500 HR chromatography. The four polysaccharides had no absorption at 280 or 260 nm in their UV spectrum, indicating the absence of protein and nucleic acid. The high performance gel permeation chromatography （HPGPC） profile showed single and symmetrically sharp peaks, indicating that they were homogeneous polysaccharides. Effects of the four polysaccharides on the free fatty acid （FFA） and lipid mobility of S180 cell membrane were investigated. The results demonstrated that all the four selenium polysaccharides have good antitumor activity, and the activities of SeAPⅠ-2 and SeAPⅡ are significantly stronger than those of SeAPⅠ-1 and SeAPⅢ （p〈0.05）.

关 键 词：木耳 多糖 抗肿瘤 分子筛 膜脂流动性 

分 类 号：R285[医药卫生—中药学]