木榄根际土壤来源的曲霉属真菌F5及其抗菌活性代谢产物  

Aspergillus sp. F5 from the rhizospheric soil of Bruguiera gymnorrhiza and its antibacterial metabolites

作　　者：何仁发[1] 李明月[1] 王娟[1] 田峰[1] 叶波平[1] 

机构地区：[1]中国药科大学生命科学与技术学院,南京210009

出　　处：《菌物学报》2010年第5期732-738,共7页Mycosystema

基　　金：教育部"新世纪优秀人才支持计划"项目(NoNCET-05-0496)

摘　　要：将一株分离自木榄根际土壤的菌株F5鉴定为曲霉属环绕组真菌成员,其在形态学特征和ITS序列上与Aspergillus ochraceopetaliformis极为相似。该菌株在改良葡萄糖蛋白胨酵母培养基(GPYM)中28℃下160r/min发酵7d后的产物具有明显的抑菌活性。利用色谱方法从菌株F5的发酵产物中分离鉴定到2种化合物:(R)-(-)-mellein(Ⅰ)和flavacol(Ⅱ),其中,flavacol对枯草芽孢杆菌的生长具有明显的抑制作用,最低抑菌浓度(MIC)为32mg/L,而mellein对金黄色葡萄球菌和枯草芽孢杆菌的生长均无明显的抑制活性。A fungus strain F5 isolated from the rhizospheric soil of Bruguiera gymnorrhiza was identified as a member of Aspergillus section Circumdati. The morphological characters and internal transcribed spacer （ITS） sequence showed that this species is closely related to A. ochraceopetaliformis. This strain can produce antibacterial metabolites when cultured in modified glucose peptone yeast medium （GPYM） under the condition of 160r/min at 28℃ for 7 days in a rotary shaker. Two compounds were isolated from the fermentation broth and mycelial extract, and they were elucidated as （R）-（-）-mellein （Ⅰ ） and flavacol （Ⅱ ）. Compound Ⅱ inhibited the growth of Bacillus subtilis with the minimum inhibitory concentration （MIC） value of 32mg/L, while the compound Ⅰ had no antibacterial activities.

关 键 词：红树植物 曲霉属环绕组 蜂曲菌素 黄曲霉素 抗菌活性 

分 类 号：Q93[生物学—微生物学]