纳米银羟基磷灰石复合涂层的生物安全性研究  

Study on bio-safety for Nano-Ag/HAp as anti-bacterial materials

作　　者：张敏[1] 施更生[1] 林海升[1] 陈再鸿[1] 史建陆 林昌健[3] 

机构地区：[1]浙江省台州医院口腔科,临海317000 [2]厦门市口腔医院正畸科,厦门361003 [3]厦门大学化学院固体表面物理化学国家重点实验室,厦门361005

出　　处：《口腔医学》2010年第9期541-543,共3页Stomatology

摘　　要：目的研究纳米银羟基磷灰石的细胞毒性。方法采用能快速、准确、敏感地评价细胞增殖率和细胞毒性的MTT比色法,以纳米羟基磷灰石为对照组,纳米银羟基磷灰石为实验组,在材料浸提液中,分别检测人牙周膜成纤维样细胞的相对增殖率。另观察细胞在扫描电镜中的形态,从而分析比较涂层表面对细胞的毒性。结果与纳米羟基磷灰石相比,纳米银羟基磷灰石涂层中的纳米银粒子直径在200～400nm时体外细胞培养显示表现出较高的细胞增殖率,细胞毒性趋于0级,显示其具有良好的生物活性。结论纳米银羟基磷灰石涂层无细胞毒性。Objective To study the cytotoxicity of a Nano-Ag/HAp composite coating on titanium surface.Methods A Nano-Ag/HAp composite coating was deposited on the surface of pure titanium by electrochemical deposition.In vitro celluar responses of the PDLF to the coating were also evaluated by MTT assay.Conventional HAp coatings and commercially pure titanium were taken as control.Results The Nano-Ag/Hap bioceramic coating has good crystallization and homogeneity on nano-scale surface morphology.The PDLF culture exhibits satisfactory bioactivity.Conclusions The Nano-Ag/HAp bioceramic coating on titanium surface has promising prospects in the future clinical application.

关 键 词：纳米银羟基磷灰石涂层 细胞毒性 MTT比色法 

分 类 号：R783.1[医药卫生—口腔医学]