霍奇金淋巴瘤与EB病毒感染的相关性研究  

Association between Epstein-Barr virus infection and Hodgkin’s lymphoma

作　　者：郑雄伟[1] 林晋[1] 林贤东[1] 陆俐丽[1] 力超[1] 

机构地区：[1]福建医科大学教学医院,福建省肿瘤医院病理科,福建福州350014

出　　处：《中国癌症杂志》2010年第8期634-637,共4页China Oncology

摘　　要：背景与目的:EB病毒(Epstein-Barr virus,EBV)感染与多种肿瘤的发生密切相关,EBV感染与霍奇金淋巴瘤(Hodgkin's lymphoma,HL)的关系较为密切,但在不同地区、性别及不同年龄之间,HL患者中EBV的检出率存在着差异,本研究通过组织芯片及原位杂交技术研究福建地区HL患者与EB病毒感染的关系。方法:在福建地区收集74例组织病理学上诊断为HL组织标本,制成组织芯片。采用原位分子杂交方法检测淋巴瘤组织中EBV编码的小RNA(EBV-encoded small RNA,EBER),确定EBV在淋巴瘤细胞中的存在,进而分析EBV感染与患者临床病理学特征的关系。结果:HL组织中EBER阳性的均为经典型HL患者,其EBV的检出率为51.4%(38/74),在HL各型中EBV的检出率以淋巴细胞消减型HL(lymphocyte depletion Hodgkin's lymphoma,LD)和混合型HL(mixed cellularity Hodgkin's lymphoma,MC)为高,7例LD中有5例阳性及28例MC有23例阳性,而结节硬化型HL(nodular sclerosis Hodgkin's lymphoma,NS)和淋巴细胞为主型HL(lymphocyte rich Hodgkin's lymphoma,LP)分别为18例有5例阳性和17例5例阳性。EBER在≤14岁儿童组中10例有9例阳性,在≥70岁老年组中8例有7例阳性,成人EBER阳性率为39.3%(22/56),两两比较差异具有统计学意义(χ2=13.4,P<0.05)。Ⅲ～Ⅳ期的HL患者EBV阳性率(76%,27/42)显著高于Ⅰ～Ⅱ期患者(33%,11/32),差异有统计学意义(χ2=7.7,P>0.05)。男性HL患者的EBER阳性率为54.7%(29/55),而女性患者阳性率为42.7%(9/21),男女比例为1.3∶1。结论:福建地区HL的发病与EB病毒的感染有一定的关系,其中以HL中MC和LD与EB病毒的关系较为密切。Background and purpose：The occurrence of many tumors is related with Epstein-Barr virus （EBV）, especially in Hodgkin’s lymphoma （HL）.But the positive rate of EBV was variable in different regions,sex and age. The aim of this study was to investigate the association of EB infection with HL in Fujian detected by tissue array and in situ hybridization. Methods：Seventy-four cases with pathologically diagnosed as Hodgkin’s lymphoma were collected and were made to a tissue microarray section. In situ hybridization was employed to detect the EBV-encoded small RNA （EBER） in tumor cells. Results：All of EBV-associated Hodgkin’s lymphoma were classical ones, 38 cases of 74 Hodgkin’s lymphomas were showed EBV positive （51.4%）, including 5 lymphocyte depletion HL （LD） （5/7）,23 mixed cellularity HL （MC） （23/28）, 5 nodular sclerosis HL （NS） （5/18） and 5 lymphocyte rich HL （LP） （5/17）, LD and MC were closely related to EBV infection. There were 9 EBER positive cases of 10 cases in children with age 14 years old,7 EBER positive cases of 8 cases in those with age 70 years old and the positive rate of EBER in adult were 39.3% （22/56）. The positive rate of EBER in children and the older was significantly higher than that in adult （χ^ 2 =13.4, P〈0.05）. The patients with clinical stage Ⅲ-Ⅳ showed higher EBV expression （76%,27/42） than those in clinical stage Ⅰ-Ⅱ （33%,11/32） （χ^ 2 =7.7, P〈0^.05）. On the other hand, the positive rate of EBER was 54.7% （29/55） in male patient and 42.7% （9/21） in female ones. Conclusion：Hodgkin’s lymphoma in Fujian was associated with EBV infection,especially the type of MC and LD.

关 键 词：霍奇金淋巴瘤 EB病毒 原位杂交 组织芯片 

分 类 号：R733.4[医药卫生—肿瘤]