肌腱细胞转化生长因子β及其受体表达：6-磷酸果糖的抑制效应(英文)  

Inhibition effect of mannose-6-phosphate on expression of transforming growth factor beta and its receptor in flexor tendon cells

作　　者：申延清[1] 夏长所[1] 马爱国[2] 

机构地区：[1]青岛大学医学院附属医院,山东省青岛市266003 [2]青岛大学医学院,山东省青岛市266003

出　　处：《中国组织工程研究与临床康复》2010年第33期6258-6261,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:转化生长因子β在肌腱愈合与粘连形成中具有重要的作用,抑制转化生长因子β及其受体表达可起到一定的防止肌腱术后粘连作用。目的:探讨转化生长因子β天然抑制剂6-磷酸果糖对兔屈趾肌腱腱鞘、腱外膜和腱内膜细胞转化生长因子β及其受体的影响。方法:取兔屈趾肌腱分离培养腱鞘、腱外膜和腱内膜细胞。3种细胞分别加入6-磷酸果糖(实验组)或不加6-磷酸果糖(对照组)培养。采用酶联免疫吸附实验定量检测转化生长因子β及其受体的表达,原位杂交和免疫组织化学观察观测转化生长因子β1的表达。结果与结论:实验组细胞转化生长因子β及其受体的表达均较对照组下降(P<0.05)。实验组3种细胞阳性转化生长因子β1mRNA表达率及细胞内转化生长因子β1mRNA表达强度均较对照组明显降低(P<0.05)。免疫组化显示加入6-磷酸果糖培养后,3种细胞转化生长因子β1表达均明显降低。BACKGROUND：Transforming growth factor beta（TGF-β） has an important role in tendon healing and adhesion formation.Inhibiting TGF-β and its receptor expression may prevent adhesions after tendon open.OBJECTIVE：To study the effects of mannose-6-phosphate,a natural inhibitor of TGF-β,on TGF-β and its receptor production in tendon sheath fibroblasts,epitenon tenocytes,and endotenon tenocytes of rabbit flexor toes.METHODS：Tendon sheath fibroblasts,epitenon tenocytes,and endotenon tenocytes were isolated from rabbit flexor tendon and cultured separately.All these cells were divided into 2 groups at random,experiment group supplemented with mannose-6-phosphate and control group without mannose-6-phosphate.The expression of TGF-β and TGF-β receptor was quantified with enzyme-linked immunosorbent assay.The expression of TGF-β1 was also assessed with in situ hybridization and immunohistochemistry.RESULTS AND CONCLUSION：The expression of TGF-β and TGF-β receptor in experiment group was significantly lower than that in control group（P 0.05）.In experimental group,the positive expression of TGF-β1 mRNA and the expression level of intracellular TGF-β1 mRNA in all tendon cells demonstrated significantly lower than those in the control group（P 0.05）.Immunohistochemical staining showed expression of TGF-β1 were significantly lower in all three types of tendon cell cultured with mannose-6-phosphate.

关 键 词：肌腱细胞 6-磷酸果糖 转化生长因子Β 转化生长因子Β受体 兔 

分 类 号：R587.2[医药卫生—内分泌]