SOD对脑缺血-再灌注损伤大鼠谷氨酸转运体功能的影响  

Influence of SOD on the function of glutamate transporter in rats with injury of cerebral ischemia-reperfusion

作　　者：任晓燕[1] 葛宝林[1] 曲忠森[1] 张玉敏[1] 

机构地区：[1]青岛大学医学院病理生理学教研室

出　　处：《中国病理生理杂志》1999年第7期601-603,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金

摘　　要：目的：探讨超氧化物歧化酶（ＳＯＤ）对脑缺血再灌注损伤（Ｉ－Ｒ）大鼠皮层、海马、纹状体谷氨酸转运体功能的影响。方法：采用大鼠３个血管夹闭、松夹复制脑Ｉ－Ｒ损伤模型。利用脑组织突触膜颗粒对［３Ｈ］－Ｌ－谷氨酸摄入量的测定及分光光度法观察ＳＯＤ对皮层海马纹状体谷氨酸转运体功能、丙二醛（ＭＤＡ）含量及ＳＯＤ活性的影响。结果：Ｉ－Ｒ组谷氨酸转运体的功能及ＳＯＤ活性明显低于对照组（Ｐ＜００５，００１），ＭＤＡ含量明显高于对照组（Ｐ＜００５，００１）。ＳＯＤ＋Ｉ－Ｒ组大鼠谷氨酸转运体的功能及ＳＯＤ活性明显高于Ｉ－Ｒ组（Ｐ＜００５，００１）；ＭＤＡ含量明显低于Ｉ－Ｒ组（Ｐ＜００５，００１）。结论：ＳＯＤ可改善Ｉ－Ｒ后脑组织谷氨酸转运体的功能，其机制可能与清除自由基有关。AIM:To investigate the effect of superoxide dismutase(SOD) on the function of glutamate transporter in rats with injury of cerebral ischemia-reperfusion.METHODS:A model of ischemia-reperfusion injury(I-R) was performed by clipping three arteries and then by releasing them to reperfuse blood into ischemic brain in rats. Glutamate transporter functions were studied by means of assay of L-glutamate uptake in synaptosomes, and malondialdehyde (MDA) contents and SOD activities in cerebral contex, hippocampus ,striatum by spectrophotometry.RESULTS:Glutamate transporter function and SOD activity in I-R group significantly decreased ( P <0 05, P< 0 01);content of MDA significantly increased( P <0 05, P< 0 01) compared with control group. Glutamate transporter function and SOD activity in SOD group significantly increased ( P <0 05, P< 0 01);MDA content significantly decreased( P <0 05, P< 0 01) compared with I-R group.CONCLUSION:SOD can improve glutamate transporter function of I-R rats. The mechanism might be related to eliminate free radicals.

关 键 词：再灌注损伤 SOD 大鼠 脑缺血 谷氨酸转运体 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学] R364.12[医药卫生—临床医学]