肝素增强血小板生成素对巨核细胞增殖的作用  

Heparin enhances the effect of thrombopietin on proliferation of megakaryocytes

作　　者：李文林[1] 石小玉[1] 周莹[1] 熊丽霞[1] 关华凤[1] 

机构地区：[1]江西医学院病理生理学教研室

出　　处：《中国病理生理杂志》1999年第7期616-618,共3页Chinese Journal of Pathophysiology

摘　　要：目的和方法：为了探讨巨核细胞增殖调控，我们研究了肝素对重组人血小板生成素（ｒｅｃｏｍｂｉｎａｎｔｈｕｍａｎｔｈｒｏｍｂｏｐｏｉｅｔｉｎ，ｒｈＴｐｏ）刺激ＢＡＬＢ／ｃ小鼠巨核系祖细胞（ＣＦＵ－ＭＫ）和人巨核细胞系－红白细胞白血病（ｈｕｍａｎｅｒｙｔｈｒｏｌｅｕｋｅｍｉａ，ＨＥＬ）细胞株增殖的作用。采用半固体和液体培养、ＣＦＵ－ＭＫ－乙酰胆碱脂酶染色和５－溴－２′－脱氧尿嘧啶核苷（５－ＢｒｄＵ）－酶联免疫吸附等方法，观察了ＣＦＵ－ＭＫ和ＨＥＬ细胞集落数以及ＨＥＬ细胞的吸光度。结果：在培养基中加入ｒｈＴｐｏ１００ｎｇ／ｍＬ和肝素２５Ｕ／ｍＬ，细胞集落和吸光度高于不加肝素对照组。ＣＦＵ－ＭＫ被扩增１５０％，ＨＥＬ细胞集落和ＨＥＬ细胞吸光度增幅分别为１０２％和１０６％。结论：肝素能增强血小板生成素对巨核细胞增殖的作用。AIM and METHOD:In order to investigate the regulation of megakaryocyte proliferation, action of heparin on stimulating the proliferation of BALB/c mice CFU-MK and human megakaryocytic lineage-HEL cells by thrombopoietin (Tpo) were studied. Semi-solid and liquid cultures, CFU-MK acetylcholinesterase staining and 5-bromo-2'-deoxy-uridine (5-BrdU)-elisa were used. CFU-MK, HEL colonies and absorbance were observed. RESULTS: In the medium treated with rhTpo 100 μg/L and heparin 2.5×10 3 U/L, the colonies and the absorbance increased as compared with control group without heparin. A combination of rhTpo 100 μg/L with heparin 2.5×10 3 U/L increased CFU-MK by 150%, HEL colonies by 102% and HEL absorbance by 106%, respectively. CONCLUSION: Heparin enhances the effect of Tpo on the proliferation of megakaryocytes.

关 键 词：肝素 血小板生成素 巨核细胞 小鼠 白血病 

分 类 号：R733.702[医药卫生—肿瘤] R973.2[医药卫生—临床医学]