蟾蜍灵诱导人肺腺癌细胞凋亡作用及其机制  

Effect of Bufalin on Inducing Cell Apoptosis and Its Mechanisms in Human Lung Adenocarcinoma Cells

作　　者：郁云龙[1] 刘云鹏[2] 王锴[1] 朱志图[1] 刑永达[1] 哈敏文[1] 

机构地区：[1]辽宁医学院附属第一医院肿瘤科,辽宁锦州121001 [2]中国医科大学附属第一医院肿瘤内科

出　　处：《肿瘤防治研究》2010年第9期1000-1003,共4页Cancer Research on Prevention and Treatment

基　　金：辽宁省教育厅2009年高等学校科研项目(2009A470)

摘　　要：目的研究中药蟾酥有效成分蟾蜍灵(bufalin)对人肺腺癌细胞的作用及其机制。方法 MTT法检测蟾蜍灵对细胞的增殖抑制作用;瑞氏-吉姆萨染色法观察细胞形态学的变化;流式细胞术分析细胞凋亡和线粒体跨膜电位;Western blot法检测Bcl-2、Caspase-3蛋白的表达。结果 (1)蟾蜍灵明显抑制A549细胞增殖,48h及72h的IC50分别为(56.14±6.72)nmol/L、(15.57±4.28)nmol/L。(2)蟾蜍灵能诱导肺癌细胞凋亡,形态学表现为出现凋亡小体及流式细胞仪检测到的亚二倍体凋亡峰。(3)蟾蜍灵可以明显降低细胞线粒体跨膜电位(ΔΨm)。(4)蟾蜍灵诱导细胞凋亡过程中,下调Bcl-2蛋白表达(P<0.01),同时活化Caspase-3。结论线粒体途径是蟾蜍灵诱导肺腺癌A549细胞凋亡的主要通路之一。Objective To study the mechanism of bufalin on inducing cell apoptosis in human lung adenocarcinoma（A549）cells.Methods The growth inhibition was analyzed by MTT assay;The morphological changes in the samples dyed in wright-Giemsa were observed using light microscope.Apoptosis and Mitochondrial membrane potential（ΔΨm）was evaluated by flow cytometry,Western blot analysis was used to evaluate the level of Bcl-2 and Caspase-3 expression.Results （1）Bufalin inhibits cell growth,and 50%inhibitory concentrations（IC50）of the agent at 48h and 72h were（56.14± 6.72）nmol/L,（15.57±4.28）nmol/L,respectively.（2）Apoptosis of A549 cells could be efficiently induced by bufalin at different concentrations.（3）A significantly reduction in the Mitochondrial membrane potential（ΔΨm）was induced by bufalin.（4）The expression of Bcl-2 protein was downregulated and Caspase-3 protein was activated during bufalin-induced apoptosis in A549 cells.Conclusion Bufalin-induced lung adenocarcinoma cells apoptosis is through mitochondria signaling pathway in A549 cells.

关 键 词：蟾蜍灵 肺腺癌 凋亡 BCL-2 

分 类 号：R734.2[医药卫生—肿瘤]