三七总皂苷对肝纤维化大鼠胶原及TGF-β_1 mRNA表达的影响  

Effects of total saponins of panax notoginseng on I type collage and TGF-β_1 in hepatic fibrosis rats

作　　者：曾文勇[1] 石小枫[1] 刘杞[1] 王志毅[1] 徐宁[1] 张丽旦[1] 

机构地区：[1]重庆医科大学附属二院感染科,重庆400010

出　　处：《胃肠病学和肝病学杂志》2010年第9期795-798,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家自然科学基金(30671867);重庆市自然科学基金(2004)

摘　　要：目的从mRNA水平观察三七总皂苷对肝纤维化大鼠Ⅰ型前胶原及TGF-β1的影响。方法用四氯化碳复合因素诱导大鼠肝纤维化模型。同时给予三七总皂苷治疗,秋水仙碱治疗组作对照,病理HE染色观察纤维化程度分期,用RT-PCR进行Ⅰ型前胶原mRNA和TGF-β1mRNA的半定量检测。结果三七总皂苷能抑制肝纤维化大鼠Ⅰ型前胶原及TGF-β1的合成表达,减轻肝纤维化程度。结论三七总皂苷具有抗肝纤维化作用,干预大鼠肝纤维化可能与减少TGF-β1含量有关。Objective To observe the effect of total saponins of panax notoginseng（SPN） on the production of collagen I and TGF-β1 in experimental hepatic fibrosis rats induced by carbon tetracloride.Methods Experimental hepatic fibrosis model was made by intracutaneous injection of CCl4 twice a week in rats for 12 times.From the first day after the CCl4 injection,SPN（60 or 30 mg/kg） were given intracutaneously once a day for 42 days.The rats were sacrificed at the end of the 43rd days.The expression of type collagen I and TGF-β1 mRNA of hepatic tissue were measured by RT-PCR method.Results The expression of type collagen I and TGF-β1 mRNA were more in liver hepatic fibrosis model group than those in SPN treatment group.Conclusion SPN inhibited the expression of the type collagen I and TGF-β1 and could slow down the progression of hepatic fibrosis.

关 键 词：三七总皂甙 肝纤维化 Ⅰ型胶原 TGF-Β1 

分 类 号：R575[医药卫生—消化系统]