胆红素致新生兔脑瘫动物模型的实验研究  

A Study on the Animal Model of Bilirubin-induced Cerebral Palsy in Newborn Rabbits

作　　者：孙叶强[1] 李晓捷[1] 吴军[1] 李永库 姜志梅[1] 李珂静[1] 李林 

机构地区：[1]佳木斯大学医学院省小儿脑瘫防治疗育中心,佳木斯154002

出　　处：《中国康复》1999年第2期65-67,共3页Chinese Journal of Rehabilitation

基　　金：黑龙江省科研课题

摘　　要：为研制胆红素致脑瘫动物模型，将新生24h内的仔兔30只分为正常组A，实验组B、C各10只。实验组B、C分别腹腔注射胆红素100、300mg／kg。正常组与实验组仔兔自然哺育45d后对其进行神经行为学检查和评分。结果：实验组B仔兔神经行为得分与正常组A相比差异无显著意义；实验组C仔兔神经行为得分与正常组A相比差异有非常显著意义；实验组C中有8只仔兔神经行为得分较高，但与正常组A相比差异仍有非常显著意义，另2只得分较低，有明显运动及姿势异常，其病理改变主要为基底节明显黄梨，镜下神经细胞受损，表现为核扭曲、消失，神经元数量明显减少，胶质细胞增生。提示腹腔注射胆红素300mg／kg能造成仔兔脑损伤并致脑瘫。In an attempt to develop an animal model of bilirubin-induced cerebral palsy, 30 newborn rabbits (age <24h) were divided into three groups (A: normal control group; B and C: experimental group, n= 10 in each). The rabbits in groups B and C were administered with bilirubin intraperitoneally 100 mg/kg and 300 mg/kg respec- tively. The neural behaviors of all animals were checked and their scores given after being fed naturally for 45 days.It was found that there was a significant difference between A and C groups in scores of neural behavior. Eight rab- bits in group C had higher scores, but there was a significant difference between groups A and C, the remaining 2 rabbits had lower scores of the behavior. abnormal movement and posture. These results suggested that bilirubin (i. p. 300 mg/kg) could cause brain damage of newborn rabbits and induce cerebral palsy.

关 键 词：胆红素 脑瘫 动物模型 新生儿黄疸 

分 类 号：R742.302[医药卫生—神经病学与精神病学] R722.170.6[医药卫生—临床医学]