18↑F-FDG PET-CT对裸鼠鼻咽癌移植瘤放疗疗效的早期评估及与Ki67表达的相关性研究  

18↑F-FDG PET-CT in early assessing the therapeutic effects of nasopharyngeal carcinoma xenograft in nude mice after radiotherapy and it＇s relativity to the expression of Ki67

作　　者：冼伟均[1] 冯彦林[1] 袁建伟[1] 张宁[2] 黄克敏[1] 贺小红[1] 袁白虹[1] 苏少弟[1] 刘艳[1] 叶秋莲[3] 

机构地区：[1]中山大学附属佛山第一人民医院核医学科,广东佛山528000 [2]中山大学附属佛山第一人民医院放疗科,广东佛山528000 [3]中山大学附属佛山第一人民医院儿科,广东佛山528000

出　　处：《国际放射医学核医学杂志》2010年第1期11-15,共5页International Journal of Radiation Medicine and Nuclear Medicine

基　　金：吴阶平医学基金（320.6750.06113）;广东省医学科学技术研究基金（A2006657）

摘　　要：目的 探讨18↑FDG PET-CT早期评估裸鼠鼻咽癌移植瘤放疗疗效的价值,并初步分析18↑F-FDG PET-CT与肿瘤细胞增殖因子Ki67表达之间的关系,为临床进一步研究提供依据.方法 将15只鼻咽癌裸鼠移植瘤动物模型随机分成3组：每组5只,对照组：行18↑F-FDG PET-CT后并处死;6 Gy放疗组：于放疗前及放疗后1d分别给予显像后处死;12 Gy放疗组：于放疗前、放疗6Gv时、放疔12Gv后第2 d及6 d时分别行18↑F-FDGPET-CT,并于最后一次显像后处死.所有裸鼠处死后取移植瘤行病理学检查及Ki67染色,分析放疗疗效与Ki67表达的相关性.结果 ①12 Gy放疗组放疗前平均T/NT值为2.17±0.31,放疗6 Gy时平均T/NT值为1.68±0.42,放疗12 Gy后2 d时平均T/NT值为1.41±0.40,放疗12 Gy后6 d时平均T/NT值为0.70±0.12,放疗前与放疗6 Gy时没有统计学差异（t=2.108,P〉0.05）;放疗12 Gy后2 d与放疗前、放疗6 Cy时比较明显减低,有统计学差异（与放疗前比较,t=2.80,P〈0.05;与放疗6 Gy 时比较,t=3.14,P〈0.05）;放疗12 Gy后6 d移植瘤的T/NT值较放疗后2 d进一步减低（与放疗后2 d比较,t=3.49,P〈0.05;与放疗前比较,t=8.01,P〈0.01）.②鼻咽癌移植瘤Ki67的表达随放疗剂量的增加明显降低,不同组别间Ki67的表达有明显差别（F=21.95,P〈0.01）：12 Gy放疗组Ki67表达显著低于对照组（t=7.145,P〈0.01）及6 Gy放疗组（t=2.384,P〈0.05）;6 Cy放疗组Ki67的表达较对照组减低同样存在统计学意义（t=4.320,P〈0.01）.③放疗前移植瘤T/NT值与Ki67表达之间无相关性.④放疗过程中移植瘤T/NT值的变化与Ki67表达水平呈正相关（r=0.532,P〈0.05）.结论 18↑F-FDG PET-CT对早期评估裸鼠鼻咽癌移植瘤的放疗疗效具有重要价值,放疗后6d是一个合适的时间点;18↑F-FDG PET-CT结合Ki67的表达变化可以反映鼻咽癌的放疗敏感性.Objective To investigate the value of 18↑F-FDG PET-CT scans on early assessing the therapeutic effects on human nasopharyngeal carcinoma （NPC） xenograft in nude mice after radiotherapy,and preliminarily analyze the relation of 18↑F-FDG PET-CT scans and the positive expression rate of Ki67, and provide a basis for further clinical studies. Methods Fifteen xenograft nude mice of NPC were randomly divided into three groups （5 nude mice per group）. Control group： all mice immediately executed after the 18↑F-FDG PET-CT scans and taken pathology; 6 Gy radiotherapy group： all mice took 18↑F-FDG PET-CT scans before and 1 d after receiving 6 Gy irradiation, and were killed immediately to take pathology; 12 Gy radiotherapy group： all mice received 12 Gy irradiation （6 Gy each time, totally 2 times）. They took 18↑F-FDG PET-CT scans before radiotherapy, after receiving the first 6 Gy irradiation, 2 d and 6 d after receiving 12 Gy irradiation respectively ,and then were executed to take pathology after the last scan. The expression of Ki67 in xenografts of different groups was detected and the relation between their changes and T/NT were analyzed.Results ①For 12 Gy radiotherapy group, the average T/NT ratios of the NPC xenograft of pre-radiotherapy, after the first 6 Gy irradiation, 2 d and 6 d after 12 Gy radiotherapy were 2.17±0.31, 1.68±0.42, 1.41±0.40,and 0.70±0.12, respectively. The average T/NT ratios were not statistically difference before and after the first 6 Gy irradiation. The average T/NT ratio of 2 d after radiotherapy decreased significantly than pre-radiotherapy （t=2.80, P〈0.05） and after the first 6 Gy irradiation （t=3.14, P〈0.05）; The average T/NT ratio of 6d after radiotherapy was decreased seriously than 2 d after radiotherapy（t=3.49, P〈0.05, compared with preradiotherapy t=8.01, P〈0.01）. ② The positive expression rates of Ki67 of NPC xenograft markedly decreased after receiving irradiation. There were significant differences in the positive expressio

关 键 词：鼻咽肿瘤 小鼠 裸 X线疗法 氟脱氧葡萄糖F18 正电子发射断层显像术 体层摄影术 X线计算机 Ki-67 抗原 

分 类 号：R73[医药卫生—肿瘤]