高产壳聚糖酶菌株的筛选及其降解壳聚糖反应初探  

FILTRATION OF BEAUVERIA BASSIANA WITH HIGH-YIELD CHITOSANASE AND ITS ACTIVITY OF HYDROLYZING CHI- TOSAN

作　　者：杜昱光[1] 方祥年[2] 白雪芳[1] 黄秀梨[2] 

机构地区：[1]中国科学院大连化学物理研究生化室,大连116023 [2]北京师范大学生物系,北京100875

出　　处：《中国海洋药物》1999年第2期24-27,共4页Chinese Journal of Marine Drugs

基　　金：国家科委"九五"攻关资助项目!(96-C03-01-10)

摘　　要：通过两轮有效的紫外线诱变，获得了２株具有高产壳聚糖酶的突变株．具有较好的稳定性。其发酵液的粗酶活性达 ０．２ Ｕ／ｍｌ，比原始保存突变菌株酶活性提高了 ８倍。并得到一定量壳寡糖。Two high chitosanase producing mutant srains mutagenized by UV were obtained. The experimental results indicated that the highest-yield mutant's chitosanase activity in the fermentation liquid was 0. 2U/ml and 8 times higher than the original strain. Some certain kinds of chitosan oligosacchrides were obtained.

关 键 词：球孢白僵菌 诱变 壳聚糖酶 壳寡糖 

分 类 号：Q939.1[生物学—微生物学] O636.1[理学—高分子化学]