宁波市90株结核分枝杆菌临床分离株MLVA基因分型研究  

Analysis on the genotyping of 90 mycobacterium tuberculosis clinical strains isolated from Ningbo with MLVA

作　　者：于梅[1] 许国章[1] 董红军[1] 车洋[1] 杨薇娜[1] 余易[1] 

机构地区：[1]宁波市疾病预防控制中心,宁波315010

出　　处：《卫生研究》2010年第5期618-620,共3页Journal of Hygiene Research

摘　　要：目的研究宁波市结核分枝杆菌的可变数目串联重复序列(VNTR)的分型及分布特征。方法随机选取宁波市结核分枝杆菌临床分离株,常规培养,提取菌体基因组DNA,通过聚合酶链反应(PCR)对15个VNTR位点进行检测,采用BioNumerics软件进行基因分型的聚类分析。结果 90株结核分枝杆菌临床分离株的VNTR位点检测结果显示出明显的基因多态性,基因分型经聚类分析,分为4个基因群(Ⅰ群、Ⅱ群、Ⅲ群、Ⅳ群),84个基因型。Ⅰ群占11.1%,含有10个基因型,Ⅱ群占17.8%,含15个基因型,Ⅲ群占65.6%,含54个基因型,Ⅳ群占5.6%,含5个基因型。结论宁波市结核分枝杆菌VNTRs基因存在明显多态性得到初步证实,至少存在4个VNTR型,主要流行型为Ⅲ型。Objective To investigate the genotyping and distribution pattern of the variable number tandem repeats（VNTR） of Mycobacterium tuberculosis clinical isolates in Ningbo with multiple-locus VNTR analysis（MLVA）.Methods M.tuberculosis clinical isolates selected from Ningbo randomly were cultured with Lowenstein-Jensen medium.The bacteria from which the genome DNA was extracted were collected.The polymorphism of VNTR locus was tested in the DNA sample with PCR respectively.The clustering of genotype was analyzed with BioNumerics.Results 15 VNTR loci of 90 M.tuberculosis clinical isolates were analyzed respectively.There were obvious polymorphisms of VNTRs.The clustering of genotype showed that these strains could be categorized into 4 gene clusters（Ⅰ,Ⅱ,Ⅲ,Ⅳ） and 84 genotypes.11.1% were clusterⅠ including 10 genotypes,17.8% were clusterⅡincluding 15 genotypes,65.6% were cluster Ⅲ including 54 genotypes,5.6% were cluster Ⅳ including 5 genotypes.Conclusion The results showed that there were obvious polymorphisms of VNTRs of M.tuberculosis clinical strains preliminarily.There were at least 4 clusters of VNTR genotype and cluster Ⅲ was the predominant prevalent strain in Ningbo.

关 键 词：结核分枝杆菌 基因分型 可变数目串联重复序列 

分 类 号：R378.911[医药卫生—病原生物学]