转生长激素基因鲤鱼的雌激素样作用研究  

Estrogenic Effect of Growth Hormone Gene Transgenic Carp on Immature Rats

作　　者：刘玉梅[1,2] 张文众[1] 雍凌[1] 贾旭东[1] 齐丽娟[1] 赵晓红[2] 李宁[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050 [2]北京联合大学应用文理学院,北京100191

出　　处：《中国食品卫生杂志》2010年第5期385-389,共5页Chinese Journal of Food Hygiene

基　　金：国家重点基础研究发展计划973计划课题(2007CB109207)

摘　　要：目的研究转生长激素基因鲤鱼是否具有类雌激素样作用。方法用啮齿动物子宫试验方法,选用19日龄未成年雌性SD大鼠,分为6个组:溶剂对照组玉米油5ml/(kgBW·d),亲本鲤鱼对照组3g/(kgBW·d),转生长激素基因鲤鱼组3g/(kgBW·d),E2(17β-雌二醇)经口低剂量组1μg/(kgBW·d),E2经口高剂量组3μg/(kgBW·d),E2经皮组3μg/(kgBW·d)。所有剂量组连续给予受试物3d(从19日龄到21日龄)。实验结束称量子宫湿重和干重,对子宫、卵巢和阴道做组织病理学检查,并测量子宫内膜上皮细胞高度。结果与溶剂对照组比较,E2经口低剂量组和转生长激素基因鲤鱼组及亲本鲤鱼对照组的子宫重量差异无统计学意义,E2经口高剂量组和E2经皮组与溶剂对照组比较差异有统计学意义(P<0.05)。结论转生长激素基因鲤鱼经口摄入未见雌激素样作用。Objective To explore the potential estrogen-like activity of growth hormone （GH） gene in transgenic carp.Method Uterotropic assay was used.Nineteen-day-old immature female rats were randomly divided into 6 groups：using corn oil 5 ml /（kg BW·d） in vehicle control group,parental carp meal 3 g /（kg BW·d） in counterpart control group,GH gene transgenic carp meal 3 g /（kg BW·d） in transgenic carp group,oral administration with 1 μg /（kg BW·d） in oral low E2 group,oral administration with E2 3 μg /（kg BW·d） in oral high E2 group,and hypodermic injection with E2 3 μg /（kg BW·d） in hypoderm high E2 group.All animal groups were given related test substances for 3 days （from the postnatal day 19 to day 21）.The wet and dry weights of uterus were measured and the histopathological changes of uterus,ovary and vagina were examined by the end of the test.In addition,morphometric measurement of endometrial epithelium was conducted for quantitative comparison.Results Comparing with the vehicle control group,significant differences for wet and dry weights of uterus were observed in oral high E2 group and hypoderm high E2 group （P〈0.05）,and no significant difference was observed in other groups.Conclusion No estrogenic effect of GH gene transgenic carp was shown in uterotropic assay.

关 键 词：啮齿动物子宫试验 转生长激素基因鲤鱼 雌激素 

分 类 号：Q789[生物学—分子生物学]