应用LAMP方法检测动物源性食品中沙门菌  

Application of LAMP to Detect Salmonella in Animal Derived Foods

作　　者：何翠华[1] 刘志强[1] 王丹云[1] 孙启明[2] 黄纪徽[1] 

机构地区：[1]海南出入境检验检疫局检验检疫技术中心,海南海口570311 [2]海口市疾病预防控制中心,海南海口570203

出　　处：《中国食品卫生杂志》2010年第5期411-414,共4页Chinese Journal of Food Hygiene

摘　　要：目的运用两种方法对动物源性食品中沙门菌进行检测,找出一种更加快速、敏感、特异、简便的检验方法。方法采集60份市售的禽、畜等生肉及60份奶制品,分别采用LAMP方法和国家标准方法GB/T4789.4—2008对沙门菌进行分离与鉴定。结果 120份采集样品中,LAMP方法检出2例,阳性率1.67%;国家标准方法检出1例,阳性率0.83%。LAMP方法的符合率为99.2%,敏感性为100%,特异性为99.2%。结论 LAMP检测法快速、特异、简便,该方法与国标法的阳性率差异无显著性。Objective Using two methods of Salmonella in animal derived foods were detected,to find a more rapid,sensitive,specific,simple testing method.Method Collected from 60 commercial poultry,livestock and other raw meat and 60 dairy products,respectively using loop-mediated isothermal amplification （LAMP） method and the national standard method GB /T 4789.4—2008 to separate and identify of Salmonella.Results In the 120 samples,LAMP method detected two cases,the positive rate of 1.67%;national standard method detected one case,the positive rate of 0.83%.LAMP method of compliance was 99.2%,a sensitivity of 100% and specificity of 99.2%.Conclusion LAMP detection method is rapid,specific,simple.This method and the positive rate of national standard was no significant difference.

关 键 词：环介导等温扩增技术 沙门菌 动物源性食品 

分 类 号：R155.5[医药卫生—营养与食品卫生学]