水稻XIOsPR10基因表达调控的初步研究  

Preliminary Study on Regulation of XIOsPR10 Gene Expression in Rice

作　　者：郭迟鸣[1] 毛倩[1] 杨晓坡[1] 郭立佳[1] 王丽娟[1] 陈亮[1] 

机构地区：[1]厦门大学生命科学学院,细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《西北植物学报》2010年第9期1721-1727,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：科技部863专题(2007AA10Z132);国家重大科技专项(2008ZX08001-001,2009ZX08009-045B);教育部重点项目(01102)

摘　　要：为探索水稻PR10蛋白在水稻抗细菌性条斑病中的作用,构建了XIOsPR10基因的植物过量表达载体p1301-XIOsPR10及RNAi表达载体pDS1301-XIOsPR10,通过农杆菌介导分别转化水稻愈伤组织,获得了相应的再生植株。经GUS检测和PCR分析,证实XIOsPR10基因以及RNAi片段分别整合到水稻再生植株基因组中;半定量RT-PCR分析显示,过量表达植株中XIOsPR10基因的表达量高于对照,而RNAi转基因植株中XIO-sPR10基因的表达被抑制。In order to study the function of rice XIOsPR10 gene which is related to the plant disease resistant reaction,we constructed plant over expression and RNAi expression vector and integrated into the genome of rice via Agrobacterium tumefaciens EHA105,respectively.The transgenic cultivars were identified by PCR and GUS gene expression detection.The analysis result showed that the transcription level of XIOsPR10 of over-expression transgenic rice was higher than that of control.In construct,XIOsPR10 gene expression was blocked in RNAi transgenic rice.

关 键 词：XIOsPR10 过量表达载体 RNAi表达载体 水稻 

分 类 号：Q789[生物学—分子生物学]