清热滋阴活血复方制剂对大鼠糖尿病牙周炎的疗效和血清IL-6的影响  

The effect of a Chinese herbal compound on rat diabetic periodontitis and serum IL-6 levels

作　　者：白广亮[1] 林志勇[1] 赵芬[1] 赵民[1] 季梅[1] 于桂娜[1] 李雪[1] 

机构地区：[1]山东大学附属省立医院,山东济南250021

出　　处：《牙体牙髓牙周病学杂志》2010年第9期518-521,530,共5页Chinese Journal of Conservative Dentistry

基　　金：山东省自然科学基金项目(2007ZRB14001)

摘　　要：目的:通过动物实验,观察清热滋阴活血中药复方制剂对大鼠糖尿病牙周炎的疗效及其对血清IL-6的影响。方法:10周龄健康雄性Wistar大鼠随机分为健康对照组(A组)、模型空白对照组(B组)和中药治疗组(C组),B组与C组采用链脲佐菌素腹腔注射(60mg/kg)并配合牙周结扎方法建立糖尿病伴牙周炎模型,然后,C组用金银花、北沙参、川芎(1∶1∶1)复方水煎剂浓缩液(1g/mL)灌胃,每天1次,每次1mL,连续使用2个月;A组和B组灌胃等体积的生理盐水。2个月后,麻醉下截取包括实验区磨牙的双侧上颌骨,制作5μm切片,HE染色后,光学显微镜观察并选择包括牙龈结缔组织和上皮组织的视野进行炎细胞计数;同时测量实验牙近中牙槽嵴顶至釉牙骨质界的距离;腹腔总静脉抽取静脉血,用ELISA方法检测血清IL-6的含量。结果:实验结束后,中药治疗组(C组)血糖较治疗前明显降低(P<0.05),但仍高于健康对照组;非治疗组(B组)血糖无改善,且较治疗前稍有升高。组织学观察,C组浸润炎细胞数、牙槽嵴顶至釉牙骨质界的距离均低于B组,但仍高于A组,三组间比较差异有统计学意义(P<0.05);血清IL-6的含量C组与A组无显著差异(P>0.05),均明显低于B组(P<0.05)。结论:清热滋阴活血中药复方制剂对糖尿病牙周炎有一定的治疗作用,可降低血清IL-6的水平。AIM： To observe the effect of a Chinese herbal compound of ＂cleaning heat nourishing yin and activating blood circulation＂ on rat diabetic periodontitis and on serum IL-6. METHODS：A total of 65 10-week-old healthy male Wistar rats were randomly divided into the control group （ Group A） , blank control group （ Group B） and the traditional Chinese medicine treatment group （ Group C）. Group B and group C received iutraperitoneal injection of streptozotocin （60mg/kg） and periodontal ligation to establish diabetes with periodontitis. Then group C was fed with a honeysuckle, north adenophora and chuanxiong compound decoction （ 1 ： 1 ： 1, 1 g/mL ） for 2 months, 1 mL per day. Group A and B were ted with nomal saline. The bilateral maxillas corresponding to the molar area were then dissected under anesthesia. After HE staining, inflammatory cells were counted and the mesial distances between the top of the alveolar ridge crest and the cemento-enamel junction border were measured. Serum levels of IL-6 were determined by ELISA. RESULTS ： After 2 months oral intake of the compound, the blood glucose levels of group C significantly decreased （ P 〈 0.05 ）, though higher than that of group A. Blood glucose in group B was not decreased and even slightly higher than that of before treatment. Both of the number of infiltrating inflammatory cells and the distance from the alveolar ridge crest to the cemento - enamel junction in group C were lower than in those group B （ P 〈 0.05 ）. Serum IL-6 levels in group C were significantly lower than in those group B （ P 〈0. 05 ）. CONCLUSION ： Our results showe that the compound is effective in treating diabetic periodontitis.

关 键 词：糖尿病牙周炎 清热滋阴活血 细胞因子 IL-6 

分 类 号：R781.4[医药卫生—口腔医学]