发酵产丁二酸过程中废弃细胞的循环利用  

Recycle of spent cells from anaerobic succinate fermentation

作　　者：白雪飞[1] 陈可泉[1] 叶贵子[1] 黄秀梅[1] 李建[1] 姜岷[1] 

机构地区：[1]南京工业大学生物与制药工程学院材料化学工程国家重点实验室,南京210009

出　　处：《生物工程学报》2010年第9期1276-1280,共5页Chinese Journal of Biotechnology

基　　金：国家重点基础研究发展计划(973计划)(No.2009CB724701);国家自然科学基金(No.20606017);材料化学工程国家重点实验室基金;江苏省"青蓝工程"资助~~

摘　　要：对厌氧发酵产丁二酸后的废弃细胞进行破壁处理,考察了以细胞水解液作为有机氮源重新用于丁二酸发酵的可行性。比较了超声破碎、盐溶、酶解3种方法破碎细胞获得的水解液作为氮源发酵产丁二酸的效果,结果表明酶解制得的细胞水解液效果最佳。以总氮含量为1.11g/L的酶解液(相当于10g/L酵母膏)作为氮源发酵,丁二酸产量可达42.0g/L,继续增大酶解液用量对耗糖、产酸能力没有显著提高。将细胞酶解液与5g/L酵母膏联用发酵36h后,丁二酸产量达75.5g/L,且丁二酸生产强度为2.10g/(L·h),比使用10g/L酵母膏时提高了66.7%。因此,厌氧发酵产丁二酸结束后的废弃细胞酶解液可以替代原培养基中50%的酵母膏用于发酵。Spent cells recovered from anaerobic fermentation by Actinobacillus succinogenes were used as nitrogen source for succinic acid production. Three methods were investigated for cell wall-breaking. The results showed that enzymatic hydrolysis was more effective for higher succinic acid yield. When the enzymatic hydrolysate of spent cells was added to reach a total nitrogen concentration 1.11 g/L （equivalent to 10 g/L yeast extract）,the succinic acid concentration was 42.0 g/L,but it increased slightly when enhancing the level of enzymatic hydrolysate. However,when 5 g/L yeast extract was supplemented with the enzymatic hydrolysate of spent cells,the succinic acid concentration reached 75.5 g/L after 36 hours and,the succinic acid productivity was 2.10 g/（L·h）,which increased by 66.7% compared with the fermentation using 10 g/L yeast extract. Therefore,enzymatic hydrolysate of spent cells could replace 50% yeast extract in the original medium for succinic acid production.

关 键 词：废弃细胞 酶水解 氮源 丁二酸 

分 类 号：Q819[生物学—生物工程]