雌二醇对HepG_2细胞载脂蛋白AⅠmRNA表达的影响  

The Effects of Estradiol on Apolipoprotein AⅠ mRNA Transcription in HepG_2 Cells

作　　者：崔丽[1] 毛用敏[2] 赵莉莉[2] 崔让庄[2] 

机构地区：[1]天津医科大学,300070 [2]天津市胸科医院心血管病研究所

出　　处：《天津医药》2010年第10期862-864,共3页Tianjin Medical Journal

摘　　要：目的:探讨雌激素对人肝癌细胞株HepG2细胞载脂蛋白(Apo)AⅠmRNA表达的影响。方法:分别将0.01、0.1、1.0、10.0、20.0μmol/L的雌二醇(E2)与体外培养的HepG2细胞共同孵育24h;选用10μmol/L的E2与HepG2细胞共同孵育0、1、6、24、48h;收集细胞提取总RNA,采用逆转录聚合酶链反应(RT-PCR)测定ApoAⅠmRNA表达水平。结果:随E2浓度的增加,HepG2细胞内ApoAⅠmRNA表达呈现先升高后降低的趋势,以10μmol/L时表达最高,与空白组及0.01、0.1、1.0μmol/L组相比差异有统计学意义(P<0.05),20μmol/L时ApoAⅠmRNA表达降低;时间刺激试验显示随刺激时间的延长,HepG2细胞中ApoAⅠmRNA表达呈现先升高后降低的趋势,刺激24h后表达水平最高,48h后下降。结论:E2可在转录水平调节ApoAⅠ基因表达,并呈现部分的剂量和时间依赖性。Objective：To observe the effect of estrogen on modulating apolipoprotein A（ApoA） Ⅰgene transcription in HepG2 cells.Methods：HepG2 cells were exposed to different concentrations of estradiol（0.01,0.1,1.0,10.0 and 20.0 μmol/L） for 24 h,respectively.HepG2 cells were incubated with estradiol at 10 μmol/L concentration for 1,6,24 and 48 h.RNA was extracted from HepG2 cells and collected at above time points.The expression of ApoAⅠmRNA was analysed by RT-PCR.Results：The level of ApoAⅠmRNA expression was increased with estradiol in a concentration-dependent manner in HepG2 cells.The level of ApoAⅠ mRNA was significantly higher in 10 μmol/L of estradiol than that in 0,0.01,0.1 and 1.0 μmol/L of estradiol.The expression of ApoAⅠmRNA was decline at 20 μmol/L.The level of ApoAⅠmRNA showed a time-dependent increase as stimulated by estradiol in time-course experiment.The expression of ApoAⅠmRNA was significantly increased at 6 h（P 0.05） and the highest at 24 h（P 0.05） compared with that of other time points.Conclusion：Estrogen may play an important role in the transcription of ApoAⅠgene.The effect of estrogen on the mRNA expression showed a concentration-dependent and time-dependent manner.

关 键 词：雌二醇 载脂蛋白A-Ⅰ 逆转录聚合酶链反应 细胞系 肿瘤 RNA 信使 

分 类 号：R735.7[医药卫生—肿瘤]