放线菌剂对黄瓜幼苗生长及叶片PPO活性的影响  

Effects of Antimicrobial Actinomyces on Growth and PPO Activity in Cucumber

作　　者：段春梅[1] 薛泉宏[1] 赵娟[2] 呼世斌[3] 陈秦[1] 王玲娜[2] 申光辉[2] 薛磊[2] 

机构地区：[1]西北农林科技大学资源与环境学院,陕西杨凌712100 [2]西北农林科技大学生命学院,陕西杨凌712100 [3]西北农林科技大学污染控制研究中心,陕西杨凌712100

出　　处：《西北农业学报》2010年第9期48-54,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家环保示范项目(财建2006-859)

摘　　要：以黄瓜幼苗为试材,采用盆栽试验研究了3种生防放线菌剂单接和与病原菌混接对黄瓜的促生作用及对黄瓜叶片PPO活性的影响。结果表明:①供试放线菌制剂对黄瓜幼苗有显著的促生作用,在接种量为1.5 g/kg、20 d时,菌剂Act12(密旋链霉菌,Streptomyces pactum)单独接种处理后,黄瓜植株总质量、根系鲜质量、根系长度、根系活力及叶面积分别较对照CK提高18.8%、225.0%、57.3%、482.0%及45.3%;Act12与黄瓜枯萎菌HK混合接种时,其各对应指标测值分别较对照HK提高78.9%、114.6%、162.6%、443.4%及50.0%。②施用菌剂后对叶片诱导酶PPO活性有一定提高,45 d时,单接Act12(1.5 g/kg)较对照增加了54.4%。③接种放线菌剂对黄瓜幼苗叶片绿色度SPDA无明显影响。以上结果表明,施用放线菌剂能显著促进黄瓜生长发育,对根系生长及活力的促进作用尤为明显,并使黄瓜产生诱导抗性。In this paper,the effect of three strains of antimicrobial actinomyces on cucumber growth and induced PPO（polyphenol oxidase） activity of cucumber leaves were studied.The results showed that,（i）The growth of cucumber was promoted by three strains of antimicrobial actinomyces.When only Act12 was inoculated after 20 days with the inoculation concentration 1.5 g/kg,the biomass,root fresh weight,root length,PPO activity and leaf area increased respectively by 18.8%,225.0%,57.3%,482.0% and 45.3% compared with the control treatment.Meanwhile,when Act12 was inoculated,the pathogen,the biomass,root fresh weight,root length,PPO activity and leaf area rose respectively by 78.9%,114.6%,162.6%,443.4% and 50.0% compared with the HK treatment inoculated with Fusarium oxysporum.（ii）The PPO activity of cucumber leaves was promoted significantly by the three strains of antimicrobial actinomyces.When only Act12 was inoculated 45 days with the inoculation concentration 1.5 g/kg,the PPO activity increased 54.4% compared with the control treatment with out inoculation.（iii）The three actinomyces had no significantly effect on SPAD value of cucumber leaves.In summary,all the three antimicrobial actinomyces could significantly promote cucumber growth and increase PPO activity,thus enhance the induced resistance.

关 键 词：放线菌菌剂 黄瓜 促生作用 PPO活性 

分 类 号：S642.2[农业科学—蔬菜学]