实时荧光定量PCR检测鼻咽癌组织中PTCH-1和SMO基因的表达  

Expression of PTCH-1 and SMO mRNA in nasopharyngeal carcinoma

作　　者：肖白玉[1] 党华[2] 甘嘉裕[2] 蔡谦 张国萍[1] 常弘[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]中山大学附属第一医院黄埔院区,广东广州510700 [3]附属第二医院,广东广州510120

出　　处：《细胞与分子免疫学杂志》2010年第10期955-958,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：广东省科技计划项目资助(2008B030301136)

摘　　要：目的:检测Hedgehog信号通路基因PTCH-1和SMO在鼻咽癌(NPC)组织中的表达及其生物学意义。方法:利用实时荧光定量PCR分别检测32例鼻咽癌组织及32例鼻咽炎组织中PTCH-1和SMO基因的表达情况。根据Livak(2-△△Ct)法进行相对定量表达分析,以鼻咽炎组织为对照,计算鼻咽癌组织中PTCH-1和SMO表达水平。结果:在所检测的全部鼻咽癌组织和鼻咽炎组织中PTCH-1和SMO都有表达。与鼻咽炎组织相比,有75%(24/32)鼻咽癌组织的PTCH-1基因表达水平下调,有68.8%(22/32)鼻咽癌组织的SMO表达水平上调,差异均具有统计学意义(P<0.05)。此外,有18.7%(6/32)鼻咽癌组织PTCH-1基因表达水平上调,有15.6%(5/32)鼻咽癌组织SMO基因表达水平下调,差异均无统计学意义(P>0.05)。结论:在鼻咽癌中SMO基因普遍具有较高的表达水平,PTCH-1基因的表达水平则相对较低,而SMO基因表达水平的上调及PTCH-1基因表达水平的下调,可引起Hedgehog信号通路在鼻咽癌中的异常激活,Hedgehog信号通路的异常激活可能与鼻咽癌的发生发展过程相关。AIM：To detect the expression of Hedgehog pathway components PTCH-1 and SMO mRNA in nasopharyngeal carcinoma （NPC）,and discuss its biological significance.METHODS：Expression of PTCH-1 and SMO mRNA was evaluated by real-time fluorescence quantitative PCR in 32 cases of NPC and 32 cases of nasopharyngitis.According to Livak （2-Ct ） relative quantitative analysis method,with nasopharyngitis as control,to calculate the expression level of PTCH-1 and SMO in NPC.RESULTS：All detected NPC and nasopharyngitis tissues had the expression of PTCH-1 and SMO.Compared with nasopharyngitis,the negative expression rate of PTCH-1 in NPC was 75% （24/32）,and the positive expression rate of SMO in NPC was 68.8% （22/32）,the differences were statistically significant （P〈0.05）.In addition,the positive expression rate of PTCH-1 in NPC was 18.7% （6/32）,and the negative expression rate of SMO in NPC was 15.6% （5/32）,the differences were not statistically significant （P〉0.05）.CONCLUSION：The expression level of SMO in NPC is generally high,but the expression level of PTCH-1 was relatively low.The down-regulate of PTCH-1 and up-regulate of SMO may cause the abnormal activation of hedgehog signaling pathway in NPC,speculated that the genesis and development of NPC may be associated with the abnormal activation of hedgehog signaling pathway.

关 键 词：鼻咽癌 PTCH-1基因 SMO基因 实时荧光定量PCR 

分 类 号：R739.62[医药卫生—肿瘤]