禽致病性大肠杆菌生物被膜的形成及其影响因素  

The biofilm formation of avian pathogenic Escherichia coli in vitro

作　　者：欧阳凤菊[1,2] 李兆利[2] 赫明雷[2] 刘慧芳[2] 司微[2] 刘思国[2] 王春来[2] 倪洪波[2] 王宇[1] 曲娟娟[1] 

机构地区：[1]东北农业大学资源与环境学院,黑龙江哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/动物细菌病研究室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2010年第9期672-676,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：兽医生物技术国家重点实验室开放基金项目(SKLVBF2008);农业部公益行业科研专项(200803054)

摘　　要：为确定影响细菌生物被膜(BF)的形成条件,本研究采用BF体外定性观察和定量粘附性检测两种方法对1株野生禽致病性大肠杆菌(E.coli)在不同环境(培养时间、培养基类型、引导载体类型、营养条件)下产生BF的差异进行了研究。结果显示野生禽致病性E.coli其宿主体外BF最适形成条件是培养时间为48h,培养基为5%TSB、引导载体为平底96孔聚苯乙烯微孔板(美国Corning Costar)。其生长周期为8h时开始起始粘附、24h形成若干微菌落、36h微菌落粘连、48h形成完整的BF、72h细菌脱落开始下一轮的BF生长。糖分和适量的无机盐都可以在一定程度上促进BF的形成和被膜内细菌的粘附性提高。该研究表明禽致病性E.coliBF的形成周期,并为抑制其形成提供了实验依据。To investigate the biofilm formation of the avian pathogenic Escherichia coli by establishing a in vitro qualitative model and quantitative adhesion test. A wild type pathogenic E. coli was cultivated in flat-bottom 96-well polystyrene microplate substratum with 5% TSB medium under different cultivation time,mediums,substratums and nutrients. Biofilm was well developed at 37 ℃ after 48 hours cultivation. The formation period included an initial adhesion to the surface at 8 hours,the development of micro-colonies at 24 hours,the conglutination of micro-colonies at 36 hours,the mature of biofilm at 48 hours,and the desquamation from the substratum,and followed by a new formation circulation. It was also showed that sugars,sodium chloride and Mg2＋ would enhance the development of the biofilm and the adherent ability of the bacteria. The results may provide a basis for clinical therapy to the bacterial biofilm disease.

关 键 词：禽致病性大肠杆菌 生物被膜 细菌粘附性 

分 类 号：S852.61[农业科学—基础兽医学]