Antigens synthesis and antibodies preparation for furazolidone and its metabolite 3-amino-2-oxazolidinone  

作　　者：Hua Ping Zhu Ting Ting Liu Bing Liu Hui Ling Yin Xiao Long Li Li Wang Shuo Wang 

机构地区：[1]Key Laboratory of Food Nutrition and Safety (Tianjin University of Science & Technology), Ministry of Education, Tianjin 300457, China

出　　处：《Chinese Chemical Letters》2010年第9期1049-1052,共4页中国化学快报（英文版）

基　　金：supports from the Ministry of Science and Technology of the People's Republic of China(No.2009ZX09502-027 and No.2009BADB9B03).

摘　　要：Two haptens of 3-[（5-amino-furan-2-ylmethylene）amino]oxazolidin-5-one（FZ-NH_2） and 3-{[（4-carboxyphenyl）methylene]-amino} -2-oxazolidinone（CPAOZ） were synthesized.For FZ-NH2,immunogens were prepared by glutaraldehyde and diazo salt methods.For CPAOZ,immunogens were connected by the methods of the active ester and mixed acid anhydride.Compared with the combination,indirect competitive enzyme-linked immunosorbent assay（ic-ELISA） was developed with coating antigen of FZ-NH2 -OVA via the glutaraldehyde method and immunogen of CPAOZ-KLH via active ester method.Fo furazolidone and its metabolite AOZ（NPAOZ as derivative）,the sensitivities（IC50） were 2.0μg/L and 2.5μg/L,limits of detection（IC15） were 0.09μg/L and 0.25μg/L,respectively.A sensitive method was developed for the simultaneous determination of furazolidone in feed and its metabolite AOZ in tissue.Two haptens of 3-[（5-amino-furan-2-ylmethylene）amino]oxazolidin-5-one（FZ-NH_2） and 3-{[（4-carboxyphenyl）methylene]-amino} -2-oxazolidinone（CPAOZ） were synthesized.For FZ-NH2,immunogens were prepared by glutaraldehyde and diazo salt methods.For CPAOZ,immunogens were connected by the methods of the active ester and mixed acid anhydride.Compared with the combination,indirect competitive enzyme-linked immunosorbent assay（ic-ELISA） was developed with coating antigen of FZ-NH2 -OVA via the glutaraldehyde method and immunogen of CPAOZ-KLH via active ester method.Fo furazolidone and its metabolite AOZ（NPAOZ as derivative）,the sensitivities（IC50） were 2.0μg/L and 2.5μg/L,limits of detection（IC15） were 0.09μg/L and 0.25μg/L,respectively.A sensitive method was developed for the simultaneous determination of furazolidone in feed and its metabolite AOZ in tissue.

关 键 词：Furzolidone AOZ ANTIGEN Antibody ELISA 

分 类 号：S859.79[农业科学—临床兽医学] TQ225.52[农业科学—兽医学]