PRDM14在非小细胞肺癌中的表达及其与临床病理因素的关系  

Relationship between the Expression of PRDM14 in Non-small Cell Lung Cancer and the Clinicopathologic Characteristics

作　　者：刘冰冰[1] 张四洋[1] 惠林萍[2] 邱雪杉[3] 崔泽实[1] 

机构地区：[1]中国医科大学实验技术中心三部,沈阳110001 [2]中国医科大学附属第四医院中心实验室,沈阳110032 [3]中国医科大学附属第一医院病理科,沈阳110001

出　　处：《中国肺癌杂志》2010年第9期867-872,共6页Chinese Journal of Lung Cancer

基　　金：辽宁省科技计划项目(No.2009415005);沈阳市科技计划项目(No.090091)资助~~

摘　　要：背景与目的锌指蛋白转录抑制因子(positive regulatory domain zinc finger protein,PRDM)是一个有关人类肿瘤形成的转录调节因子家族,在细胞分化和恶性变中发挥重要作用。PRDM14是PRDM家族的成员之一。本研究的目的是检测PRDM14在非小细胞肺癌组织中的表达情况并探讨其与非小细胞肺癌的临床病理因素的关系。方法采用免疫组织化学方法检测70例非小细胞肺癌标本和7例癌旁组织中PRDM14的表达。采用Western blot方法检测42例非小细胞肺癌组织和癌旁肺组织中PRDM14蛋白的表达。结果 7例癌旁肺组织中PRDM14弱表达,在70例非小细胞肺癌组织标本中,有8例为PRDM14阴性表达(11.43%),9例为PRDM14弱阳性表达(12.86%),36例PRDM14阳性表达(51.43%),有17例PRDM14强阳性表达(24.29%),PRDM14的表达情况与非小细胞肺癌的分化程度(P=0.046)和组织学类型(P=0.047)有关,PRDM14在高分化腺癌、鳞癌中表达最高,在中分化腺癌、鳞癌中表达次之,在低分化腺癌、鳞癌表达最低,PRDM14在腺癌中的表达高于鳞癌。Western blot结果表明PRDM14的蛋白在癌旁肺组织和肺腺癌、鳞癌组织的表达水平存在显著差异,PRDM14在非小细胞肺癌组织中的表达水平高于癌旁肺组织(P<0.001),而且与非小细胞肺癌的分化程度有关(P=0.017),在高、中分化腺癌、鳞癌中的表达高于在低分化腺癌、鳞癌中的表达。结论 PRDM14在癌旁肺组织中低表达,在非小细胞肺癌组织中高表达,其表达水平与非小细胞肺癌的分化、组织学类型有关,可能在非小细胞肺癌的发生发展中发挥作用。Background and objective The positive regulatory domain proteins（PRDM） are family of transcriptional regulation related to the formation of human tumor factor and play key roles in the cell differentiation and malignant transformation.PRDM14 is a member of the PRDM family.The aim of this study is to detect the expression of PRDM14 in non-small cell lung cancer（NSCLC） tissues,and analyze its relationship with clinicopathologic characteristics of NSCLC.Methods PRDM14 expression was detected in 70 NSCLC specimens and 7 paracancerous tissues using the immunohistochemistry（SP method）.The PRDM14 protein expression was determined in 42 NSCLC specimens and 42 paracancerous tissues by Western blot.Results Among 70 NSCLC specimens,8 specimens showed weak expression of PRDM14（11.43%,8/70）,62 specimens showed moderate to strong staining of PRDM14（88.57%,62/70）,whereas 7 paracancerous specimens showed weak staining extent.PRDM14 expression level was positively correlated with differentiation（P=0.046） and histological type（P=0.047）.The positive cytoplasmic expression of PRDM14 in highly differentiated NSCLC,the low expression of PRDM14 in poorly differentiated NSCLC.The results of Western blot showed that there were significant difference between the two groups（P〈0.001）;expression of PRDM14 was conspicuous in NSCLC specimens but low in paracancerous tissues.PRDM14 expression level was positively correlated with differentiation（P=0.017）.The positive cytoplasmic expression of PRDM14 in highly differentiated NSCLC,the low expression of PRDM14 in poorly differentiated NSCLC.Conclusion The high expression of PRDM14 in NSCLC is associated with differentiation and histological type.The PRDM14 may play an important role in the development of NSCLC.

关 键 词：肺肿瘤 PRDM14 组织学分型 分化 

分 类 号：R734.2[医药卫生—肿瘤]