昆虫病原线虫的培养及其对两种天牛的致病性测定  

Culturation of the Entomogenous Nematode and Determination of It's Pathogenility to Two Species of the Longicorn

作　　者：王忠文[1] 廖咏梅[1] 张桂英[1] 杨崇振 李钟明 

机构地区：[1]广西大学农学院

出　　处：《广西农业生物科学》1999年第2期109-113,共5页Journal of Guangxi Agricultural and Biological Science

基　　金：广西区教育厅资助

摘　　要：经筛选，Ｆ号培养基的线虫产量最高，１０ｄ开始可以看见大量子代线虫，２０ｄ线虫量最大，达到６０．７万头／ｇ，以液体培养基先培养共生细菌２ｄ，后和线虫悬浮液一起接种入线虫培养基，能获得最好的效果。人工培养的线虫在室内对光绿桔天牛（Ｃｈｅｌｉｄｏｎｉｕｍａｒｇｅｎｔａｔｕｍ）的致死率为４８ｈ达８６．０％。在田间施用时，由于虫道口小，虫道长而复杂，致死率稍低，为７０％左右；在室内对蔗根土天牛（Ｄｏｒｙｓｔｈｅｎｅｓｇｒａｎｕｌｏｓｕｓ）幼虫无致病作用。Three media were selected to culture the entomogenous nematode Steiernema glaseri which is a potential biotogic agent for insect composed of eggs 20%,lard 5%,peptone 1%,sponge 8% and watter 50% was the best for the reproduce of the nematode and the yield reached 6 07×10 5 per gram medium after 18 days cultured.It could get better reproduction when the symbiotic bacteria was cultured in the liquid medium 2 days before innoculated the nematode.The test showed that the lethal effiacy of the nematode to Chelidonium argentatum was 86 0% and had no effect to Dorysthenes granulosus from laboratory experiments.The effiacy of the nematode to the former was 70% from field trial.

关 键 词：昆虫病原线虫 人工培养 天牛 致病性 测定 

分 类 号：S476.19[农业科学—农业昆虫与害虫防治]