成熟培养时间对水牛卵母细胞体外成熟的影响  

Effect of Culture Time on the in Vitro Maturation and Fertilization of Buffalo Oocytes

作　　者：李青[1] 阳年生[1] 卢晟盛[1] 

机构地区：[1]广西大学动物科技学院

出　　处：《广西农业生物科学》1999年第2期117-119,共3页Journal of Guangxi Agricultural and Biological Science

摘　　要：采用ＴＣＭ１９９＋１０％ＯＣＳ为成熟培养液、以Ｔｙｒｏｄｅ｀ｓ改良液为受精液对水牛卵母细胞作体外成熟培养和受精，比较不同成熟培养时间（２４ｈ和３０ｈ）对水牛卵母细胞成熟率和受精率的影响。其结果为，水牛卵母细胞在成熟培养２４ｈ和３０ｈ后，其成熟率分别为６６．５％和６７．２％，受精率分别为５７．５％和４９．０％，在统计学上成熟率及受精率均无显著差异（Ｐ＞０．０５）。表明水牛卵母细胞的成熟培养时间可为２４ｈ和３０ｈ。The objectives of the present study were to examine the effect of oocyte culture time on the maturation and cleavage rates. Buffalo oocytes cultured in CO 2 incubator in TCM199+10%OCS for 24 h or 30 h were fertilized in Tyrode's medium or stained for microscopy. The maturation rate of the cultured oocytes was similar between the two groups (66 5% vs 67 5%, P >0 05), and the cleavage rate was 57 5% and 49 0% respectively ( P> 0.05). These results indicate that buffalo oocytes can be matured in vitro for either 24 h or 30 h under the present experimental conditions.

关 键 词：水牛 体外成熟 体外受精 成熟率 受精率 

分 类 号：S823.83[农业科学—畜牧学]