曲古菌素A对人胃癌细胞SGC-7901中组蛋白H3乙酰化水平及细胞凋亡的影响  

Trichostatin A increases histone H3 acetylation and promotes apoptosis in gastric cancer cell line SGC-7901

作　　者：杨桐树[1] 赵婷[1] 李云龙[2] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院检验科,黑龙江省哈尔滨市150081 [2]哈尔滨医科大学第二临床医学院普外科,黑龙江省哈尔滨市150086

出　　处：《世界华人消化杂志》2010年第23期2469-2471,共3页World Chinese Journal of Digestology

基　　金：哈尔滨医科大学第二临床医学院博士科研基金资助项目;No.BS2008-05

摘　　要：目的:探讨组蛋白去乙酰化酶(HDAC)抑制剂曲古菌素A(TSA)对胃癌细胞系SGC-7901中组蛋白H3乙酰化水平及细胞凋亡的影响.方法:流式细胞仪检测细胞凋亡率,免疫细胞化学法检测75μg/LTSA干预前胃癌细胞系SGC-7901中乙酰化组蛋白H3的表达.结果:TSA干预后胃癌细胞系SGC-7901中乙酰化组蛋白H3的表达水平明显升高,乙酰化组蛋白H3阳性细胞数从1.12±1.06上升至35.43±6.05,与未干预相比两者表达有显著性差异(P<0.01),流式细胞仪分析显示细胞凋亡率增加到22.13%±3.49%.结论:TSA可诱导细胞凋亡,促进胃癌细胞系SGC-7901中乙酰化组蛋白H3的表达.AIM：To investigate the effects of trichosttain A（TSA）,a histone deacetylase inhibitor,on apoptosis in hunman gastric cancer cell line SGC-7901.METHODS：After SGC-7901 cells were incubated with 75 μg/L TSA for 48 h,cell apoptosis was measured by flow cytometry,and the expression of acetylated histone H3 was detected by immunohistochemistry.RESULTS：TSA could significantly inhibit proliferation and induce apoptosis of SGC-7901 cells.The expression level of acetylated histone H3 was higher in SGC-7901 cells treated with TSA（75 μg/L for 48 h） than in control cells（35.43 ± 6.05 vs 1.12 ± 1.06）.The apoptosis rate was significantly different between SGC-7901 cells treated with TSA（75 μg/L for 48 h） and control cells（P 0.05）.CONCLUSION：TSA can induce SGC-7901 cell apoptosis possibly by up-regulating the expression of acetylated histone H3.

关 键 词：胃肿瘤 曲古菌素A 细胞凋亡 乙酰化组蛋白H3 SGC-7901 

分 类 号：R735.2[医药卫生—肿瘤]