ATRA联合奈达铂对人肝癌细胞株Huh-7增殖与凋亡的影响  

ATRA in combination with nedaplatin inhibits cell proliferation but promotes apoptosis in human hepatoma cell line Huh-7

作　　者：刘景[1] 徐晶 丁剑午[1] 

机构地区：[1]南昌大学第二附属医院肿瘤科江西省分子医学重点实验室,江西省南昌市330006 [2]永新县人民医院内科,江西省吉安市343400

出　　处：《世界华人消化杂志》2010年第24期2538-2544,共7页World Chinese Journal of Digestology

摘　　要：目的:探讨化疗药物全反式维甲酸(ATRA)与奈达铂(nedaplatin)单独及联合应用对人肝癌细胞株Huh-7增殖与凋亡的影响.方法:选取不同浓度的ATRA(1×10-4、1×10-5、1×10-6mol/L)、奈达铂(1、2、5mg/L)并选用ATRA1×10-5mol/L联合不同浓度奈达铂(1、2、5mg/L)分别作用于肝癌细胞24、48、72h;另外,通过预实验筛选有效终浓度的ATRA(1×10-5mol/L)+奈达铂(1mg/L)作为联合处理组作用于肝癌细胞24、48、72h;采用倒置显微镜观察细胞形态学改变,MTT法观察ATRA、奈达铂及两药联合对Huh-7细胞增殖的影响;应用流式细胞仪分析其对细胞凋亡率的影响.结果:不同浓度的ATRA(1×10-4、1×10-5、1×10-6mol/L)和奈达铂(1、2、5mg/L)单独作用于Huh-7细胞均有明显的抑制作用,该作用随时间的延长、浓度的增加而逐渐增强(P<0.01);两药联合表现为协同作用,与单药相比具有显著性差异(P<0.01).Huh-7细胞经ATRA1×10-5mol/L、NDP1mg/L及两药联合作用48h后,流式细胞仪检测凋亡凋亡率分别为28.49%±0.6%,42.57%±1.03%,55.35%±1.30%,单药组与联合组比较具有显著性差异(均P<0.01).提示ATRA(1×10-5mol/L)+奈达铂(1mg/L)联合作用时具协同效应.结论:ATRA与奈达铂对人肝癌细胞株Huh-7均有抑制增殖和促进凋亡的作用,且与单药比较,ATRA联合奈达铂能够明显提高肝癌细胞对化疗的敏感性.AIM：To observe the effects of all-trans retinoic acid（ATRA）and nedaplatin,alone or in combination,on cell proliferation and apoptosis in human hepatoma cell line Huh-7,and to determine whether the two drugs in combination have a synergistic effect in treating hepatocarcinoma.METHODS：After Huh-7 cells were incubated with different concentrations of ATRA（10-4,10-5 and 10-6 mol/L）and nedaplatin（1,2 and 5 mg/L）,alone or in combination,for 24,48 and 72 h,cell morphology changes were observed using an inverted microscope;cell proliferation was evaluated by MTT assay;and apoptosis was evaluated by flow cytometry.RESULTS：Both ATRA and nedaplatin could markedly inhibit cell proliferation in a timeand concentration-dependent manner（all P 0.01）.ATRA and nedaplatin in combination had a signifi cant synergistic effect on cell proliferation compared with ATRA or nedaplatin alone（both P 0.01）.Both ATRA and nedaplatin promoted the apoptosis of Huh-7 cells.The apoptosis rate was significantly lower in Huh-7 cells treated with ATRA and nedaplatin alone for 48 h than in those treated with the two drugs in combination（28.49% ± 0.6%,42.57% ± 1.03% vs 55.35% ± 1.30%,both P 0.01）,suggesting a synergistic effect of combined nedaplatin and ATRA on the apoptosis of Huh-7 cells.CONCLUSION：Both ATRA and nedaplatin can inhibit cell proliferation and promote apoptosis in human hepatoma cell line Huh-7.ATRA combined with nedaplatin has a significant synergistic effect on the proliferation and apoptosis of Huh-7 cells.

关 键 词：全反式维甲酸 奈达铂 HUH-7细胞 细胞增殖 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]