水通道蛋白4在外燥小鼠肺组织表达的实验研究  

Experimental Study of Aquaporin 4 Expression of Mice Lung in External-dryness

作　　者：丁建中[1] 贺尚荣[1] 张燕翔[1] 李军川[2,3] 王明明[4] 

机构地区：[1]长江大学医学院,湖北荆州434023 [2]长江大学附属第一医院 [3]荆州市第一人民医院病理科,湖北荆州434023 [4]湖北中医药大学,湖北武汉430065

出　　处：《长江大学学报（自科版）（下旬）》2010年第3期1-2,28,共3页Journal of Yangtze University

基　　金：湖北省自然科学基金项目(2009CDB289)

摘　　要：目的:观察外燥对小鼠肺组织水通道蛋白4(AQP4)表达的影响。方法:无特定病原体(SPF)级昆明种小鼠60只随机分为常温常湿组、常温燥组、温燥组和凉燥组,每组15只。免疫组化法检测各组第6、12、18天小鼠肺组织AQP4阳性表达并进行图像分析。结果:各组AQP4阳性表达部位多在肺泡上皮处。与外燥相关的3个组第12、18天AQP4蛋白表达阳性率持续下降(P<0.05),伴明显肺部炎性病变。结论:外燥伤肺、耗津之时,伴肺泡液细胞内外转运相关的AQP4蛋白表达的下降,提示与其病机相关。Objective:To observe the effects of external dryness on the expression of Aquaporin 4(AQP4) in mice alveoli.Methods:60 Kunming mice(SPF) were divided into group A(normal),group B(normal temperature and External-dryness),group C(Warm-Dry),group D(Cool-Dry),15 mice/group.The expression of AQP4 were tested in days 6,12 and 18.Results:The positive expressing of AQP4 were located in the membrane edge of alveoli.Compared with group A,the positive rate of AQP4 in group B,C and D were continuously reduced in days 12 and 18(P<0.05)as the evident inflammations of lung.Conclusion:The decrease in AQP4 expression was related to the mechanisms of how the external-dryness brings about damage to the lung.

关 键 词：外燥 鼠 肺 水通道蛋白4(AQP4) 

分 类 号：R228.5[医药卫生—中医基础理论]