去甲斑蝥素对人乳腺癌细胞MCF-7的杀伤效应及其可能机制  

The killing effect and possible mechanism of norcantharidin on mammalian cells

作　　者：梁福佑[1] 戎煜[1] 陈莉[1] 高鼎成[1] 戴杰 安威[1] 

机构地区：[1]首都医科大学细胞生物学系

出　　处：《西北大学学报（自然科学版）》1999年第3期262-264,共3页Journal of Northwest University（Natural Science Edition）

基　　金：北京市自然科学基金

摘　　要：用１０μｇ／ｍＬ去甲斑蝥素（ＮＣＴＤ）作用于人乳腺癌细胞，以ＭＴＴ法、流式细胞仪、台盼蓝排斥法和透射电镜等方法分别观察ＮＣＴＤ对癌细胞的增殖抑制作用及对细胞周期和细胞凋亡的影响。结果表明：ＮＣＴＤ对乳腺癌细胞的杀伤作用有时间和剂量依赖性，药物作用２４ｈ后，Ｇ１期细胞减少，Ｇ２＋Ｍ期细胞明显增多，凋亡细胞百分比升高并可见到凋亡细胞的形态学变化。In order to observe the killing effect of an antitumor drug:norcantharidin(NCTD) ,10 μg/mL NCTD was treated on mammalian cells. The cell cycle changes and morphological characteristics of apoptosis cells were found 24 h later by FACS analysis and ultascope. The results showed that the inhibition of cell proliferation were maintained in a time and dose dependent manner in some extent. A higher rate change of G 2+M phage and apoptosis cells and lower rate change of G 1 phage cells were examined after NCTD was treated on mammalian cell for 24 hours.

关 键 词：去甲斑螯素 乳腺癌细胞 MCF-7 杀伤效应 

分 类 号：R737.905[医药卫生—肿瘤]