LYVE-1在子宫颈鳞状细胞癌中的表达与癌组织淋巴管生成及淋巴道转移关系研究  

The expression of LYVE-1 in cervical squamous cell carcinoma and the relationship between tumor lymphangiogenesis and cervical squamous cell carcinoma metastasis through the lymphatic system

作　　者：张一真[1] 俞薇薇[1] 徐娟 衷筱琴[1] 鄢敏[1] 况晓东[1] 

机构地区：[1]南昌大学医学院病理学教研室,江西南昌330006 [2]宜春医学院

出　　处：《中国妇幼保健》2010年第27期3869-3871,共3页Maternal and Child Health Care of China

基　　金：江西省卫生厅基金资助〔062010〕

摘　　要：目的:观察子宫颈鳞状细胞癌中淋巴管的分布特点及增殖状态,研究肿瘤淋巴管生成与淋巴道转移的关系。方法:收集80例子宫颈鳞状细胞癌及其相应的正常宫颈组织标本,采用免疫组化双标记技术,运用淋巴管特异标记物LYVE-1检测微淋巴管密度(MLD),LYVE-1与Ki-67进行双标免疫组化染色检测淋巴管增殖活性。结果:宫颈鳞状细胞癌肿瘤边缘区淋巴管多呈管样扩张状,而肿瘤其他区域多呈闭索状。宫颈鳞状细胞癌组织中MLD较正常宫颈组织显著性增高(P<0.05),MLD在肿瘤边缘区、低分化宫颈鳞状细胞癌组、伴淋巴结转移组中显著增高,在临床分组间差异无统计学意义。宫颈鳞状细胞癌中淋巴管内皮细胞Ki-67指数较正常宫颈组织增高(P<0.05),肿瘤边缘区Ki-67阳性表达的微淋巴管比肿瘤其他区域、有淋巴结转移组比无淋巴结转移组有显著性增高(P<0.05)。结论:宫颈鳞状细胞癌组织中存在淋巴管生成,淋巴管主要分布在肿瘤边缘区且淋巴管内皮细胞增殖活性增高。淋巴管密度与宫颈鳞状细胞癌分化程度、淋巴道转移有关,与临床分期无明显关系。检测淋巴管密度和增殖状态对预测淋巴道转移可提供一定的理论依据。Objective：To investigate the distribution patterns and proliferative activity of lymphatic vessels in cervical squamous cell carcinoma （CSCC） through double-labeling immunohistochemistry with antibody to LYVE-1 as a marker of lymphatic vessel-specific marker, explore the rule of tumor lymphatic genesis and CSCC metastasis through the lymphatic system.Methods：80 cases of CSCC and the corresponding normal tissue were obtained, microlymphatic density（MLD） was evaluated by immunohistochemistry with LYVE-1 antibodies respectively.The Ki-67 expression at lymphatic was detected by double-labeling immunohistochemistry.Results：The lymphatic vessel at the tumor border of CSCC had large and open lumina, while those at other portions of CSCC often had a reticular architecture with numerous tiny and ill-defined lumina. The MLD in CSCC was notable higher than that in the normal tissue; the MLD were notable higher at the tumor border of CSCC, the group of low differentiation degree and the group with lymph node metastasis, there was no distinct difference of the number of MLD between stage Ⅰand Ⅱ CSCC. The Ki-67 index of lymphatic vessels endothelial in CSCC was notable higher than that in the normal tissue.The Ki-67 index of the edge area of CSCC was higher than other areas（P〈0.05）, the group with lymph node metastasis was significantly higher.Conclusion：There are newly born lymphatic vessels in CSCC. The most lymphatic vessels live at tumor border of CSCC and the proliferative activity of lymphatic endothelial cells is increased. There is no definite correlation between clinical FIGO stage of CSCC with the MLD of CSCC, while the MLD of CSCC is related to the differentiation degree of CSCC and the with lymph node metastasis. The detection of MLD and proliferative activity of lymphatic vessels in CSCC may be valuable in predicting lymphatic metastasis.

关 键 词：宫颈鳞状细胞癌 微淋巴管密度 淋巴管生成 LYVE-1 Ki-67 淋巴道转移 

分 类 号：R737.33[医药卫生—肿瘤]