山东半岛地区汉族人群HLA-A、B、DRB1高分辨等位基因及单体型多态性的研究  

The distribution of HLA-A,B,DRB1 alleles and haplotypes of Han unrelated stem cell donors in Shandong Peninsula,China

作　　者：刘晓华 冯智慧 胡彬 李长缨 迟晓云 孙旭敏 焦淑贤 

机构地区：[1]青岛市中心血站青岛市输血医学研究所,山东青岛266071

出　　处：《中国输血杂志》2010年第8期592-595,共4页Chinese Journal of Blood Transfusion

基　　金：山东省医药卫生发展计划项目(2005034);青岛市南区科技发展计划基金资助项目(2009-5-21-GG)

摘　　要：目的研究山东半岛地区汉族人群HLA-A、B、DRB1等位基因多态性的分布特征。方法应用聚合酶链反应-直接测序分型(polymerase chain reaction sequence-based typing,PCR-SBT)法和序列特异性寡核苷酸探针杂交技术(Polymerase chain reaction and sequence-specific olignucleotide probe hybridizations,PCR-SSOP)高分辨试剂,对山东半岛地区865名无血缘关系的汉族健康人群HLA-A、B、DRB1进行基因分型。结果检出HLA-A、B、DRB1等位基因分别有33、73、43种,经统计分析这3个基因座分布均符合Hardy-Weinberg平衡定律(P>0.05),A*3001-B*1302-DRB1*0701(7.61%)和A*3303-B*4403-DRB1*1302(1.674%)单体型是山东半岛地区汉族人群最常见单体型。结论山东半岛地区汉族人群HLA-A、B、DRB1基因座单体型分布具有高度的遗传多态性且有其自身分布特点。本研究获得的的HLA-A、B、DRB1基因座单体型分布数据及相关遗传参数资料,为HLA在人类学、免疫遗传学、法医学和其它的组织器官移植方面的应用和科学研究提供和积累了基础资料。Objective To analyze the polymorphism and haplotypes of HLA-A,B,DRB1 loci in Han population of Shandong Peninsula.Methods PCR-SBT and PCR-SSOP were applied to determine HLA-A,B,DRB1 genotypes of 865 unrelated healthy individuals from Shandong peninsula.And the frequencies of alleles and haplotypes were calculated via Algorithm3.1.Results A total of 33 HLA-A alleles,73 HLA-B alleles and 43 HLA-DRB1 alleles were detected.And A^＊3001-B^＊1302-DRB1^＊0701（7.61%） and A^＊3303-B^＊4403-DRB1^＊1302（1.674%） were the most common haplotypes.Conclusion The distribution of alleles and haplotypes of Shandong Han population is close to that of northern Chinese population.And the results of our study could provide more genetic information to anthropology,transplantation,and disease association studies.

关 键 词：人类白细胞抗原(HLA) 高分辨基因分型 单体型 山东半岛 汉族 

分 类 号：R457.11[医药卫生—治疗学]