CPE感染力滴定法评价磁载体复合光敏剂对模型病毒的灭活效果  

Evaluation of the inactivation efficiency of the composite magnetic carrier photosensitizer by CPE determination with model virus as indicator

作　　者：张腾霄[1] 王斌[1] 高文运[2] 高婧 

机构地区：[1]绥化学院生物与食品工程系,黑龙江绥化152061 [2]西北大学生命科学学院 [3]国家微检测系统工程技术研究中心

出　　处：《中国输血杂志》2010年第8期606-610,共5页Chinese Journal of Blood Transfusion

基　　金：绥化学院杰出青年科学基金项目(SJ08003)

摘　　要：目的建立定量测定病毒感染力的方法,并用该法分析评价2种磁载体光敏剂(EMP-AA,EMP-AB)对指示病毒的灭活效率。方法利用模型病毒Pseudorabies virus(PRV)感染宿主细胞,产生的致病变效应结合ReedMuench法测定灭活处理前后病毒滴度TC ID50值并计算滴度变化量,同时从光照时间、单位灭活体系中光敏剂复合磁性微球的效用浓度及光照时间及强度方面优化病毒灭活条件,考察材料及光化学法对几种血液成分中指示病毒的灭活效果。对灭活条件筛选优化,并用优化后的灭活病毒体系进行灭活实验,结果材料EMP-AA和EMP-AB灭活效果良好,能使病毒滴度下降4 Lg以上。结论病毒感染力测定法适合光化学灭活效果的评价,测定结果能更真实的反映材料的灭活效能。Objective To establish a method to determine TCID50,which can indicate the infection ability of virus,and then to use the method to evaluate the inactivation efficiency for model virus.Methods Pseudorabies virus was used as the model virus to infect the cultivated cells,and then cytopathic effect occurred.The experiment based on cytopathic effect and Reed Muench statistical method was used to determine the TCID50 of the virus,before and after the inactivation treatment,then the quantitative decrease was calculated.The inactivation conditions were optimized from three main factors： irradiation time,irradiation intensity and concentration of composite magnetic particles.Two kinds of material and different way of photochemical treatment was assayed to detect their influences to virus inactivation efficiency.Results After optimization,EMP-AA and EMP-AB played an excellent performance,and the decline value of PRV titer was more than 4 Lg TCID50.Conclusion The method for detecting the infection ability of virus is suitable to evaluate the efficiency of photochemical inactivation,and the result can actually reflect the capability of the material at the domain of virus inactivation.

关 键 词：病毒灭活 光敏剂 光化学 感染力滴定 磁性微球 

分 类 号：R511[医药卫生—内科学] R454.2[医药卫生—临床医学]